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Nucleosides Separation by Reversed-Phase, Single-column Chromatography with Recycle Lag
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Resumo(s)
The purpose of this project is to experimentally validate a newly developed chromatographic platform for (bio)purification based on a novel single-column device that mimics the operation of multicolumn chromatography through ingenious management and recycling of mixed fractions. The newly developed platform shares the benefits of simulated-moving-bed (SMB) chromatography in that it not only gives significantly higher yields of purer product, but also enables processing more feed and thereby increasing the overall throughput. However, the proposed process uses a single chromatographic column.
The process is based on the realization that the periodic state of an SMB process can be mimicked by a single-column chromatographic process with a recycle lag of (N−1)τ time units, where N is the number of columns of the equivalent SMB unit and τ is the switching interval (time interval between consecutive switches of the inlet and outlet ports). The recycle lag is implemented in practice by means of a special type of plug-flow tube (recycle piston) that includes a moving piston to compensate for the difference between inlet and outlet flow rates. The proper operation of inlets and outlets of such device implements an approximate “first in, first out” method of organizing and manipulating the fractions of fluid collected from the chromatography column, where the oldest (first) amount fluid, or “head” of the fraction, is the first to exit the plug-flow tube.
Using the separation of nucleoside mixtures by reversed-phase chromatography as a case study, it is demonstrated that the single-column chromatographic process can mimic the operation of recent multicolumn capture and polish processes designed for the efficient separation and purification of biomolecules.
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Palavras-chave
Chromatography SMB Plug-Flow Single-Column Recycle Piston