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Contribuição para o estudo da caracterização molecular de uma colónia de Glossina morsitans morsitans Westwood 1850, (Díptera: Glossinidae).
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Com esta dissertação, pretendemos contribuir para um melhor conhecimento dos aspectos entomológicos da Glossina morsitans morsitans Westwood, 1850, existente no insectário da Unidade de Entomologia Médica do Instituto de Higiene e Medicina Tropical (UEM/IHMT), e para o início do estudo da sua análise molecular.
A dissertação está dividida em quatro partes e inclui ainda uma lista de siglas, abreviaturas, índices de figuras, tabelas, gráficos e quadros, assim como anexos que se julgou pertinente incluir.
A INTRODUÇÃO, consiste na primeira parte do trabalho, fazendo-se nela uma abordagem sobre o historial da mosca tsétsé, a nagana, a Tripanossomíase Humana Africana (THA) e a contribuição portuguesa para o seu estudo e também para o combate tanto da doença como para o combate do vector.
Referimo-nos aos aspectos da sistemática, da distribuição geográfica e de alguns factores condicionantes, como o habitat, o comportamento trófico das glossinas, a morfologia, a fisiologia e a bioecologia dos vectores.
São também abordados alguns aspectos de interesse em medicina dos agentes patogénicos e as suas relações inter hospedeiros vertebrados e modo de transmissão.
Ainda neste capítulo referimos a importância da metodologia na luta contra os vectores, integrada no controlo das tripanossomíases africanas.
Na segunda parte, MATERIAL E MÉTODOS, descrevemos o modelo experimental utilizado na nossa amostra, destacando a origem, as características e as técnicas de manutenção.
São descritos os materiais, as técnicas e as metodologias utilizadas para a extracção e amplificação do Ácido Desoxirribonucleico (ADN) genómico, seguindo-se a purificação e sequenciação dos produtos amplificados.
Na terceira parte, RESULTADOS, apresentamos os diferentes estudos realizados e resultados obtidos, durante a formação da colónia de glossinas que são utilizadas como amostra para o estudo, podendo apresentar a relação sex ratio, o número de glossinas adultas eclodidas macho e fêmea, assim como o número de pupas.
Ainda neste capítulo apresentamos a análise molecular das amostras e a sua sequenciação.
Na quarta parte, descrevemos as CONCLUSÕES, gerais do trabalho, onde se
demonstra que o sex ratio das Glossinas morsitans morsitans, criadas em colónia é
idêntico ao existente no seu habitat natural e que a sua diversidade haplotipica é
extremamente baixa.
A última parte, é constituída pelas REFERÊNCIAS BIBLIOGRÁFICAS consultadas para esta dissertação
With this essay we try to contribute for a better knowledge of the entomological aspects pertaining to the Glossina morsitans morsitans Westwood, 1850, existing in the UEM/IHMT insectariums, and the beginning of the study of its molecular analysis. The essay is divided in four parts and includes a list of initialisms, abbreviations, figure indexes, tables and graphics as well as annexes that were deemed important to include. The INTRODUCTION is the first part of this paper, and on it, an approach is made about the Tsetse fly, the “nagana”, the Human African Trypanosomiasis (HAT) and the historical Portuguese contribution for its study and also for the combat not only against the disease but also against its vector. We refer the systematics aspects, the geographic distribution and some conditioning factors such as, habitat glossinae’s trophic behaviour, the morphology, the physiology and the vectors bioecology. Some interesting aspects for medicine are also approached, in what concerns the pathogenic agents and their vertebrate hosts as well as means of transmission. Still in this chapter we mention the importance of methodology on the struggle against the vectors, integrated on the control of African trypanosomiasis.On the second part, MATERIAL AND METHODS, we describe the experimental method that it was used on our specimen, highlighting the origin, thr characteristics and maintenance techniques. It is also described the materials, techniques and methodologies that were used for the extraction and amplification of the genomic DNA (Deoxyribonucleic Acid), following it up with the purification and sequencing of the amplified products. On the third part, RESULTS, we present the different studies that were done and the results obtained, during the formation of the glossinae’s colony that is used as specimen for the study, being able to present the sex ratio as well as the number of hatched adult glossinae, male and female, and the number of pupae. Still on this chapter we present the specimens molecular analysis and its sequencing. On the fourth part, we mention the general CONCLUSIONS taken from this work, were it is demonstrated that the Glossina morsitans morsitans sex ratio, bred in colony, is identical to the existing one on its natural habitat and that its haplotipical diversity is extremely low. The last part consist on the BIBLIOGRAPHIC REFERENCES consulted for this essay.
With this essay we try to contribute for a better knowledge of the entomological aspects pertaining to the Glossina morsitans morsitans Westwood, 1850, existing in the UEM/IHMT insectariums, and the beginning of the study of its molecular analysis. The essay is divided in four parts and includes a list of initialisms, abbreviations, figure indexes, tables and graphics as well as annexes that were deemed important to include. The INTRODUCTION is the first part of this paper, and on it, an approach is made about the Tsetse fly, the “nagana”, the Human African Trypanosomiasis (HAT) and the historical Portuguese contribution for its study and also for the combat not only against the disease but also against its vector. We refer the systematics aspects, the geographic distribution and some conditioning factors such as, habitat glossinae’s trophic behaviour, the morphology, the physiology and the vectors bioecology. Some interesting aspects for medicine are also approached, in what concerns the pathogenic agents and their vertebrate hosts as well as means of transmission. Still in this chapter we mention the importance of methodology on the struggle against the vectors, integrated on the control of African trypanosomiasis.On the second part, MATERIAL AND METHODS, we describe the experimental method that it was used on our specimen, highlighting the origin, thr characteristics and maintenance techniques. It is also described the materials, techniques and methodologies that were used for the extraction and amplification of the genomic DNA (Deoxyribonucleic Acid), following it up with the purification and sequencing of the amplified products. On the third part, RESULTS, we present the different studies that were done and the results obtained, during the formation of the glossinae’s colony that is used as specimen for the study, being able to present the sex ratio as well as the number of hatched adult glossinae, male and female, and the number of pupae. Still on this chapter we present the specimens molecular analysis and its sequencing. On the fourth part, we mention the general CONCLUSIONS taken from this work, were it is demonstrated that the Glossina morsitans morsitans sex ratio, bred in colony, is identical to the existing one on its natural habitat and that its haplotipical diversity is extremely low. The last part consist on the BIBLIOGRAPHIC REFERENCES consulted for this essay.
Descrição
Palavras-chave
Parasitologia médica Glossina morsitans westwood Biologia molecular Caracterização molecular Tripanosoma brucei
Contexto Educativo
Citação
Editora
Instituto de Higiene e Medicina Tropical