ITQB: CLDMB - Master Theses
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- Live cell biosensors for detection and quantification of viral pathogensPublication . Ribeiro Fernandes, Ana Raquel"Every year, thousands of human lives are lost due to viral pathogen infections, namely Zika and Chikungunya. Currently, no vaccines or antivirals are available to prevent and treat these infections. This current and imperative medical necessity is detained by the urgent need for reliable tools to study these viruses in both fundamental and applied research. To tackle the presented problems, we developed, (i) a platform relying on a cell-based approach for viral detection and (ii) a virus-based tool for antiviral screenings. For the cell-based approach, split- GFP with prevented transcomplementation by single – embedment in an eglin c molecule (eGFP11) or intein cyclization (cyGFP11) – or dual distortions – using coiled-coils (ccGFP10+ccGFP11) – was used.(...)"
- Chimeric lentiviral vectors for gene therapy- Improving transduction efficiencyPublication . Thome Cabral, Elisa"Lentiviral vectors (LV) are excellent gene transfer tools enabling the transduction of proliferative and non-proliferative cells and the long-term transgene expression. However, LV manufacture is a high cost process characterized by low transducing competent particles yields. This work aimed to address these challenges by 1) enhancing LV transduction efficiencies, and by 2) assisting the development of constitutive LV producer cell lines for continuous, reproducible, and low-cost LV manufacture(...)"