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Enzymatic Hydrolysis Systems Enhance the Efficiency and Biological Properties of Hydrolysates from Frozen Fish Processing Co-Products
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Orientador(es)
Resumo(s)
Co-products from the frozen fish processing industry often lead to financial losses. Therefore, it is essential to transform these co-products into profitable goods. This study explores the production of fish protein hydrolysates (FPH) from three co-products: the heads and bones of black scabbardfish (Aphanopus carbo), the carcasses of gilthead seabream (Sparus aurata), and the trimmings of Nile perch (Lates niloticus). Four enzymatic hydrolysis systems were tested: an endopeptidase (Alcalase, A), an exopeptidase (Protana, P), two-stage hydrolysis with an endopeptidase followed by an exopeptidase (A + P), and a single stage with endo- and exopeptidase (AP). The results show that combined enzymatic treatments, especially single-stage Alcalase and Protana (AP), achieved high protein yields (80%) and enhanced degrees of hydrolysis (34 to 49%), producing peptides with lower molecular weights. FPH exhibited significant antioxidant activity, in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, with EC50 values below 5 mg/mL. Additionally, AP hydrolysates demonstrated over 60% angiotensin-converting enzyme (ACE) inhibition at 5 mg/mL, indicating potential antihypertensive applications. Antidiabetic and anti-Alzheimer activities were present, but at relatively low levels. AP hydrolysates, especially from gilthead seabream, proved to be the most promising. This study highlights the value of fish co-products as sources of functional peptides, contributing to waste reduction, and their potential applications in food, agriculture, and nutraceuticals.
Descrição
This work was developed within the scope of the “BLUE BIOECONOMY INNO-
VATION PACT” (Project No. C644915664-00000026) funded by NextGenerationEU, under the incentive of the “Agenda for Business Innovation” of the recovery and resilience plan (PRR).
© 2024 by the authors. Licensee MDPI, Basel, Switzerland
Palavras-chave
Alcalase anti-hypertensive antioxidant bioactive compounds molecular weight distribution Protana sequential hydrolysis simultaneous hydrolysis zero waste Pharmaceutical Science Drug Discovery Pharmacology, Toxicology and Pharmaceutics (miscellaneous) SDG 12 - Responsible Consumption and Production SDG 9 - Industry, Innovation, and Infrastructure