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Investigation Of The Bioactive And Functional Properties Of Novel Exopolysaccharides
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Os exopolissacarídeos (EPS) são macromoléculas promissoras com grande potencial para
o desenvolvimento de produtos sustentáveis de base biológica em vários setores. Os ambien-
tes marinhos, com as suas condições únicas e pouco exploradas, constituem fontes valiosas de
microrganismos capazes de produzir EPS. Estes polímeros, especialmente aqueles que contêm
açúcares raros, ácidos urónicos e grupos sulfatados, destacam-se pela sua elevada solubili-
dade, bem como pelas suas propriedades antioxidantes, fotoprotetoras e gelificantes, tor-
nando-os ideais para aplicações nas indústrias cosmética, de saúde e alimentar.
O objetivo deste estudo parte por investigar as propriedades funcionais e biológicas de
cinco EPS com composições distintas. O EPS RA19, rico em fucose, foi o único avaliado quanto
às suas propriedades funcionais, demonstrando um comportamento não-Newtoniano e
shear-
thinning, uma forte capacidade de emulsificação com óleo de parafina e a aptidão para formar
filmes estáveis, com ou sem plastificante, além de formar géis com Fe²⁺. Estas características
tornam-no adequado para aplicação em cosméticos e entrega de fármacos. No entanto, as
suas propriedades biológicas foram limitadas à fotoproteção em queratinócitos, sem apresen-
tar atividades antioxidantes ou cicatrizantes significativas.
O EPS SC4 demonstrou um excelente desempenho nas atividades fotoprotetora, antio-
xidante e cicatrizante, destacando o seu potencial para uso em cosméticos e engenharia de
tecidos. O EPS AB5 evidenciou uma atividade fotoprotetora significativa, enquanto o EPS RD5,
mostrou uma capacidade notável na cicatrização de feridas. Por fim, o EPS Mo169, apesar de
não ter demonstrado propriedades fotoprotetoras ou cicatrizantes, revelou uma forte ativi-
dade antioxidante, sugerindo a sua aplicação na mitigação do stress oxidativo.
Exopolysaccharides (EPS) are promising macromolecules with significant potential for developing sustainable bio-based products across various sectors. Marine environments, with their unique conditions, serve as valuable but underexplored sources of novel EPS-producing microorganisms. EPS featuring rare sugar, uronic acid moieties, and sulfation are particularly attractive due to their enhanced solubility, antioxidant activity, photoprotection, and gelling capabilities, making them ideal for applications in cosmetics, healthcare, and food industries. This study investigates the functional and biological properties of EPS RA19, a fucose- rich polymer. This polymer displayed non-Newtonian shear-thinning behavior, strong emulsi- fication with paraffin oil, and the ability to form stable films with and without plasticizer, and Fe²⁺-crosslinked gels, making it suitable for cosmetic and drug delivery applications. However, its biological activity was limited to photoprotection in keratinocytes, showing no antioxidant and wound healing activity. Moreover, the bioactivity of four other EPS with previously demonstrated interesting functional properties was investigated. EPS SC4 exhibited superior photoprotective, antioxi- dant, and wound-healing properties, highlighting its potential in cosmetics and tissue engi- neering. EPS AB5 showed significant photoprotective activity, while EPS RD5, rich in uronic acids, demonstrated strong wound-healing capabilities. Finally, EPS Mo169, while lacking pho- toprotective or wound-healing properties, showed strong antioxidant activity, suggesting its use in oxidative stress reduction.
Exopolysaccharides (EPS) are promising macromolecules with significant potential for developing sustainable bio-based products across various sectors. Marine environments, with their unique conditions, serve as valuable but underexplored sources of novel EPS-producing microorganisms. EPS featuring rare sugar, uronic acid moieties, and sulfation are particularly attractive due to their enhanced solubility, antioxidant activity, photoprotection, and gelling capabilities, making them ideal for applications in cosmetics, healthcare, and food industries. This study investigates the functional and biological properties of EPS RA19, a fucose- rich polymer. This polymer displayed non-Newtonian shear-thinning behavior, strong emulsi- fication with paraffin oil, and the ability to form stable films with and without plasticizer, and Fe²⁺-crosslinked gels, making it suitable for cosmetic and drug delivery applications. However, its biological activity was limited to photoprotection in keratinocytes, showing no antioxidant and wound healing activity. Moreover, the bioactivity of four other EPS with previously demonstrated interesting functional properties was investigated. EPS SC4 exhibited superior photoprotective, antioxi- dant, and wound-healing properties, highlighting its potential in cosmetics and tissue engi- neering. EPS AB5 showed significant photoprotective activity, while EPS RD5, rich in uronic acids, demonstrated strong wound-healing capabilities. Finally, EPS Mo169, while lacking pho- toprotective or wound-healing properties, showed strong antioxidant activity, suggesting its use in oxidative stress reduction.
Descrição
Palavras-chave
Exopolysaccharides (EPS) Functional properties Biological properties Fucose Sulfate Applications