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Otimização da etapa de lavagem das fibras no processo de moagem húmida, por hidrólise enzimática
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O presente trabalho tem como objetivo aumentar a recuperação de proteína e amido do processo moagem de húmida, através da otimização da etapa de lavagem das fibras pela adição de uma hidrólise enzimática. O aumento da extração destes produtos teria um impacto significativo na rentabilidade do processo.
Como tal, foi estudada a influência de diferentes complexos multienzimáticos na libertação do amido e proteína retido na suspensão. Ensaios laboratoriais, com um tempo de incubação de 3 horas e uma temperatura de 50ºC, permitiram um aumento da percentagem de amido separado e um aumento da percentagem de proteína separada da fibra com a mistura E3+E4. Estas enzimas catalisam a reação hidrólise da fibra, permitindo a extração do amido livre e ligado à parede celular que fica retido na fibra, aumentando a eficiência da etapa de lavagem.
Foi ainda estudado qual a etapa do sistema de lavagens mais adequada para realizar a hidrólise enzimática. Um ensaio laboratorial com amostras de suspensão do processo permitiu concluir que é possível separar mais 1% de amido da fibra com o substrato/suspensão da 5ª lavagem (etapa meio/final do sistema) do que com o substrato suspensão 2ª lavagem (passo inicial).
Outro objetivo deste trabalho é avaliar a influência/redução da humidade da fibra à saída da prensa, de uma hidrolise enzimática com E2, sem incubação, na etapa anterior. Assim, foi conduzido um ensaio industrial com a adição de 1,2L/h na sexta lavagem da fibra, tendo-se observado uma redução da humidade da suspensão. As enzimas vão reduzir a capacidade da fibra de reter água, aumentando a eficiência da etapa de desidratação. Uma suspensão menos húmida preciso de uma menor secagem, reduzindo os custos energéticos desta operação.
The objective of this study is to increase the recovery of protein and starch in the wet milling process by optimizing the fiber washing stage through the addition of enzymatic hydrolysis. The influence of different multi-enzymatic complexes on the release of starch and protein retained in the suspension was studied. Laboratory assays, with an incubation time of 3 hours at a temperature of 122 °F (50 °C), resulted in a increase in starch separation, and a increase in protein separation from the fiber. These enzymes catalyze the hydrolysis of the fiber, enabling the extraction of both free starch and starch bound to the cell wall retained in the fiber, thereby increasing the efficiency of the washing stage. Additionally, the most suitable stage in the washing system for enzymatic hydrolysis was investigated. A laboratory assay using suspension samples from the process concluded that it is possible to separate 1% more starch from the fiber with the substrate/suspension from the 5th washing stage (intermediate/final stage of the system) compared to the substrate/suspension from the 2nd washing stage (initial stage). Another objective of this study was to evaluate the influence of enzymatic hydrolysis on the reduction of fiber moisture at the outlet of the press, using E2 without incubation in the previous stage. To achieve this, an industrial trial was conducted by adding 1.2 L/h of enzyme in the sixth fiber washing stage, resulting in a reduction in suspension moisture. The enzymes reduce the fiber's capacity to retain water, thereby increasing the efficiency of the dehydration stage. A less moist suspension requires less drying, reducing the energy costs of this operation.
The objective of this study is to increase the recovery of protein and starch in the wet milling process by optimizing the fiber washing stage through the addition of enzymatic hydrolysis. The influence of different multi-enzymatic complexes on the release of starch and protein retained in the suspension was studied. Laboratory assays, with an incubation time of 3 hours at a temperature of 122 °F (50 °C), resulted in a increase in starch separation, and a increase in protein separation from the fiber. These enzymes catalyze the hydrolysis of the fiber, enabling the extraction of both free starch and starch bound to the cell wall retained in the fiber, thereby increasing the efficiency of the washing stage. Additionally, the most suitable stage in the washing system for enzymatic hydrolysis was investigated. A laboratory assay using suspension samples from the process concluded that it is possible to separate 1% more starch from the fiber with the substrate/suspension from the 5th washing stage (intermediate/final stage of the system) compared to the substrate/suspension from the 2nd washing stage (initial stage). Another objective of this study was to evaluate the influence of enzymatic hydrolysis on the reduction of fiber moisture at the outlet of the press, using E2 without incubation in the previous stage. To achieve this, an industrial trial was conducted by adding 1.2 L/h of enzyme in the sixth fiber washing stage, resulting in a reduction in suspension moisture. The enzymes reduce the fiber's capacity to retain water, thereby increasing the efficiency of the dehydration stage. A less moist suspension requires less drying, reducing the energy costs of this operation.
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Enzimas Recuperação Lavagem Desidratação Fibra Amido