A carregar...
Publicação
Microalgae processing towards biorefinery routes: processing of Spirulina for phycocyanin extraction and purification
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.2 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
Actualmente, uma das principais alternativas aos pigmentos sintéticos utilizados na indústria são os pigmentos naturais provenientes de microrganismos fotossintéticos. A Arthropira (Spirulina) platensis é considerada uma das principais fontes de ficocianina, um pigmento proteico, com ampla aplicabilidade na indústria devido às suas propriedades funcionais como antioxidante e anti-inflamatório.
A procura por pigmentos de origem natural tem vindo a aumentar rapidamente nos últimos anos. Prevê-se que este mercado cresça com uma Taxa de Crescimento Anual Composta (CAGR) superior a 5% até 2026, sendo que o mercado dos pigmentos alimentares estima-se que cresça 12,4 % entre 2019 e 2027. Neste sentido têm-se desenvolvido e optimizado processos de extracção de ficocianina proveniente de Arthrospira seca. Não existindo, no entanto, um estudo extenso relativo à extracção de ficocianina proveniente de Arthrospira fresca.
No presente estudo foram optimizados os processos à escala laboratorial e piloto referentes ao tratamento térmico de Arthrospira fresca para estabilização de ficocianina e moagem da biomassa de Arthrospira fresca, foi ainda desenvolvido um protocolo de quantificação de ficocianina. Os resultados deste trabalho revelaram a eficácia do tampão fosfato de potássio na extracção e estabilização de ficocianina (CPC), foi possível observar que um tratamento térmico realizado a “T” ºC promoveu a estabilidade e estendeu a vida útil do extracto de CPC. Verificou-se que a extracção sem moagem é o processo mais vantajoso e que o salting out mostra-se como um método de purificação eficiente. Denotando este uma recuperação de 96% e um ganho de concentração por parte da ficocianina superior a 139%.
Natural pigments from photosynthetic micro-organisms have stood out as one of the main alternatives to synthetic dyes and pigments. Arthropira (Spirulina) platensis is considered to be one of the main sources of phycocyanin, a protein pigment with great applicability in the industry due to its functional properties as both an antioxidant and an anti-inflammatory. The demand for natural pigments has been growing rapidly in recent years, with its market expected to grow at a Compound Annual Growth Rate (CAGR) of 5% by the year 2026, the food pigments market itself is expected to grow at a CAGR of 12,4 % between 2019 and 2027. As such, extraction processes for phycocyanin from dried Arthrospira were developed and optimized. Meanwhile, the extracting process for phycocyanin from fresh Arthrospira has not yet been extensively investigated to this day. In the present study, it was developed and optimized, the laboratory and pilot-scale processes associated with the thermal treatment of fresh Arthrospira for phycocyanin stabilization and the milling of fresh Arthrospira biomass. Additionally, a protocol for the quantification of phycocyanin was also developed. In this study, the potassium phosphate buffer was shown to be effective in extracting and stabilizing phycocyanin (CPC). The heat treatment performed at “T” ºC enhanced both the extract's stability and shelf life successfully. Performing an extraction without milling was found to be the most effective extracting method, with salting out proving to be an efficient purification method. The salting out method resulted in a pure and concentrated CPC extract with a recovery yield of 96% and a concentration gain of 139%.
Natural pigments from photosynthetic micro-organisms have stood out as one of the main alternatives to synthetic dyes and pigments. Arthropira (Spirulina) platensis is considered to be one of the main sources of phycocyanin, a protein pigment with great applicability in the industry due to its functional properties as both an antioxidant and an anti-inflammatory. The demand for natural pigments has been growing rapidly in recent years, with its market expected to grow at a Compound Annual Growth Rate (CAGR) of 5% by the year 2026, the food pigments market itself is expected to grow at a CAGR of 12,4 % between 2019 and 2027. As such, extraction processes for phycocyanin from dried Arthrospira were developed and optimized. Meanwhile, the extracting process for phycocyanin from fresh Arthrospira has not yet been extensively investigated to this day. In the present study, it was developed and optimized, the laboratory and pilot-scale processes associated with the thermal treatment of fresh Arthrospira for phycocyanin stabilization and the milling of fresh Arthrospira biomass. Additionally, a protocol for the quantification of phycocyanin was also developed. In this study, the potassium phosphate buffer was shown to be effective in extracting and stabilizing phycocyanin (CPC). The heat treatment performed at “T” ºC enhanced both the extract's stability and shelf life successfully. Performing an extraction without milling was found to be the most effective extracting method, with salting out proving to be an efficient purification method. The salting out method resulted in a pure and concentrated CPC extract with a recovery yield of 96% and a concentration gain of 139%.
Descrição
Palavras-chave
Microalgae Spirulina Biomass Stabilization Heat Treatment Biorefinery