A carregar...
Publicação
Recuperação de proteínas e alcaloides dos meios de adoçamento de Lupinus albus
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 1.48 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
O tremoço branco, Lupinus albus, é do ponto de vista nutricional uma excelente fonte de fibra e
proteína sendo por isso considerado um superalimento, contudo, a presença de alcaloides impede que
este seja consumido de forma segura. A remoção destas substâncias leva à perda de proteínas destas
sementes. Assim o objetivo deste projeto consiste no estudo da separação das proteínas e dos
alcaloides removidos no processo de adoçamento para utilização na indústria alimentar, no que diz
respeito às proteínas de origem vegetal, e obtenção de substâncias (alcaloides) que podem ser chave no
desenvolvimento de novos fármacos pela sua utilização como sintão quiral na síntese de moléculas
complexas.
A dicarboximetilcelulose (DCMC) é um polímero recentemente desenvolvido para a adsorção de
proteínas do vinho branco, que mostrou ter uma elevada eficiência quando comparado com outros
derivados da celulose, pelo que seria espectável que permitisse a separação anteriormente mencionada.
Deste modo, sintetizou-se o polímero DCMC para avaliar a sua capacidade em separar, por adsorção,
proteínas e alcaloides de meios aquosos de adoçamento do tremoço branco. Para tal foram estudados
diferentes sistemas em que se fez variar o valor de pH, não se tendo obtido resultados satisfatórios.
Sintetizou-se, a partir deste polímero (DCMC), um MIP (Molecularly Imprinted Polymer) utilizando a
lupanina como template, e testou-se a sua capacidade de adsorver seletivamente a lupanina,
separando-a dos restantes componentes da mistura. Apesar de não se ter obtido um resultado
satisfatório desta forma, a descarboxilação do MIP obtido melhorou substancialmente o seu
desempenho mostrando-se uma opção promissora.
The white lupine, Lupinus albus, is nutritionally an excellent source of fiber and protein and therefore is considered a superfood, however, the presence of alkaloids prevents it from being consumed safely. The removal of these substances leads to the loss of proteins from these seeds, so the objective of this project is to study the separation of the proteins and alkaloids removed in this process for use in the food industry, regarding proteins of plant origin, and to obtain substances (alkaloids) that may be key in the development of new drugs as chiral sintons for synthesis of complex molecules. Dicarboxymethylcellulose is a polymer recently developed for the adsorption of white wine proteins, which has shown to be highly efficient when compared to other cellulose derivatives, so it would be expected to allow the previously mentioned separation. In this way, the DCMC polymer was synthesized to assess its ability to separate, by adsorption, proteins, and alkaloids from aqueous sweetener media in the white lupine. To accomplish this, different systems were studied in which the pH value were varied, these yielded no satisfactory results. A MIP (Molecularly Imprinted Polymer) was synthesized from this polymer (DCMC) using lupanine as a template and its ability to selectively adsorb lupanine was tested, separating it from the remaining components of the mixture, despite not obtaining a satisfactory result in this way, the decarboxylation of the obtained MIP substantially improved its performance, proving to be a promising option.
The white lupine, Lupinus albus, is nutritionally an excellent source of fiber and protein and therefore is considered a superfood, however, the presence of alkaloids prevents it from being consumed safely. The removal of these substances leads to the loss of proteins from these seeds, so the objective of this project is to study the separation of the proteins and alkaloids removed in this process for use in the food industry, regarding proteins of plant origin, and to obtain substances (alkaloids) that may be key in the development of new drugs as chiral sintons for synthesis of complex molecules. Dicarboxymethylcellulose is a polymer recently developed for the adsorption of white wine proteins, which has shown to be highly efficient when compared to other cellulose derivatives, so it would be expected to allow the previously mentioned separation. In this way, the DCMC polymer was synthesized to assess its ability to separate, by adsorption, proteins, and alkaloids from aqueous sweetener media in the white lupine. To accomplish this, different systems were studied in which the pH value were varied, these yielded no satisfactory results. A MIP (Molecularly Imprinted Polymer) was synthesized from this polymer (DCMC) using lupanine as a template and its ability to selectively adsorb lupanine was tested, separating it from the remaining components of the mixture, despite not obtaining a satisfactory result in this way, the decarboxylation of the obtained MIP substantially improved its performance, proving to be a promising option.