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Pacientes com cancro da bexiga necessitam de monitorização frequente e contínua para a deteção da progressão ou recorrência da doença. Atualmente, a urina é cada vez mais utilizada como a amostra padrão, mas a sua recolha requer visitas constantes dos pacientes a unidades clínicas e, apesar de não invasiva, envolve desafios logísticos relacionados com o armazenamento e transporte das amostras. Estas limitações dificultam uma monitorização frequente e reduzem o conforto do paciente.
Neste trabalho, desenvolvemos e validámos um sistema prático de auto-recolha de urina em casa, através de um filtro de seringa, integrado num kit fácil de usar. Para facilitar o transporte das amostras, avaliámos a entrega por drone como alternativa ao transporte convencional para o laboratório. O sistema foi otimizado para a retenção de proteínas, reprodutibilidade e compatibilidade com protocolos de trabalho de espectrometria de massa de alta resolução.
As análises comparativas mostraram que o kit fornece perfis proteómicos consistentes, com recuperação de proteínas reprodutível entre replicados técnicos. O transporte por drone não introduziu ruido detetável, e tanto as amostras transportadas por drone como as retidas no laboratório preservaram proteínas-chave e vias biológicas relevantes para o cancro da bexiga. Estes resultados demonstram que o sistema proposto permite uma recolha e transporte de urina fiáveis e cómodo para o paciente.
Bladder cancer patients require frequent and continuous monitoring for the detection and management of progression or recurrence. Currently, urine is increasingly being used as the standard sample but requires patient visits to clinical facilities and, despite being non-invasive, involve logistical challenges related to sample storage and transport. These limitati-ons hinder frequent monitoring and reduce patient comfort. In this work, we developed and validated a practical at home urine self-collection system using syringe based 10 kDa molecular weight cut-off membranes, integrated into a user frien-dly kit. To facilitate sample transport, we evaluated drone based delivery as an alternative to conventional laboratory transport. The system was optimized for protein retention, reprodu-cibility, and compatibility with high resolution mass spectrometry workflows. Comparative analyses showed that the kit provides consistent proteome profiles, with reproducible protein recovery across technical replicates. Drone transport did not introduce detectable bias, and both drone transported and laboratory retained samples preserved key proteins and biological pathways relevant to bladder cancer. These findings demonstrate that the proposed system enables reliable, patient friendly urine collection and transport.
Bladder cancer patients require frequent and continuous monitoring for the detection and management of progression or recurrence. Currently, urine is increasingly being used as the standard sample but requires patient visits to clinical facilities and, despite being non-invasive, involve logistical challenges related to sample storage and transport. These limitati-ons hinder frequent monitoring and reduce patient comfort. In this work, we developed and validated a practical at home urine self-collection system using syringe based 10 kDa molecular weight cut-off membranes, integrated into a user frien-dly kit. To facilitate sample transport, we evaluated drone based delivery as an alternative to conventional laboratory transport. The system was optimized for protein retention, reprodu-cibility, and compatibility with high resolution mass spectrometry workflows. Comparative analyses showed that the kit provides consistent proteome profiles, with reproducible protein recovery across technical replicates. Drone transport did not introduce detectable bias, and both drone transported and laboratory retained samples preserved key proteins and biological pathways relevant to bladder cancer. These findings demonstrate that the proposed system enables reliable, patient friendly urine collection and transport.
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Palavras-chave
Proteomics Syringe filters Bladder Cancer Label-Free Mass Spectrometry Urine Sample