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Mass spectrometry-based proteomics analysis of whole cell and secreted extracellular vesicles of four diffuse large B-cell lymphoma cell lines
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Resumo(s)
DISCUSSION: Here we implemented an experimental and computational workflow to study the protein
expression profiles of small extracellular vesicles from in vitro cultured DLBCL cell lines. Our main
question was whether the proteomes of small extracellular vesicles could be used to segregate
the prognostic cell of origin subtypes of DLBCL. We selected 4 DLBCL cell line models, the DB and
HT cells representing germinal center b cell like (GCB) and RIVA and OCI-ly3 cells as activated b
cell like (ABC) DLBCLs. We cultured cells considering suppliers instructions to make sure our
results are comparable to others using the same models. We tried to truthfully report our
experimental conditions and sEVs purification protocol to prevent possible misinterpretation of data. To our knowledge this is the first high accuracy, global, quantitative proteomics study to
comprehensively compare the proteomes of sEVs derived from DLBCL cells. We considered the
expression of other biomolecules in sEVs. However, proteins are main effectors of encoding
genes and among the most frequently therapeutically targeted biomolecules. We decided to
take advantage of recent developments in mass spectrometry instruments147 and data analysis
strategies127 to investigate DLBCL sEVs proteomes.
Descrição
Palavras-chave
B-cell Lymphoma Cell Lines Proteomics Analysis Mass Spectrometry