Publicação
In vitro and in silico Dissolution and Permeation Assessment
| datacite.subject.fos | Engenharia e Tecnologia::Engenharia Química | pt_PT |
| dc.contributor.advisor | Ricardo, Ana | |
| dc.contributor.advisor | Paiva, Ana | |
| dc.contributor.author | Lopes, Maria Inês Queimado de Carvalho Duarte | |
| dc.date.accessioned | 2020-06-16T09:29:30Z | |
| dc.date.available | 2020-06-16T09:29:30Z | |
| dc.date.issued | 2017-03-24 | |
| dc.date.submitted | 2017 | |
| dc.description.abstract | New chemical entities (NCEs) under development tend to be progressively more poorly water soluble. As conventional dissolution tests are not representative of in vivo conditions and thus not predictive of its in vivo behavior, formulation of these orally administered drug products is often compromised. The design of a biorelevant dissolution method reflects the physiological conditions in the gastrointestinal (GI) tract, possessing a biological discriminative power given e.g. by the increased solubilization of drug molecules by bile salts and lecithin, which is of significant importance when evaluating the dissolution behavior of poorly soluble drugs. Moreover, there has been an increasing trend in the pharmaceutical industry to use mechanistic models to complement in vitro data that are an inexpensive and fast way of assisting the formulation process. The present work aims at using a biorelevant dissolution methodology to support drug product development, employing USP Apparatus 2 and different formulations (enteric and nonenteric polymers, different binders and granule sizes) of tablets of spray dried dispersions (SDDs) of Itraconazole (ITZ), a poorly water-soluble drug. SDDs, tablets and the reference commercial product dissolution were assessed in biorelevant media and a biorelevant pH shift was performed. Also, an attempt was made to simultaneously evaluate dissolution and in vitro permeation of ITZ, using the reverse dialysis membrane methodology. Finally, an in silico model describing dissolution phenomena of amorphous active pharmaceutical compounds (APIs) was developed. Crystalline ITZ solubility in biorelevant media could not be assessed, since it was below the limit of quantification of the employed method. SDDs could not be properly tested in USP Apparatus 2 due to the characteristic poor wettability of these powders, that led to powder floating. The potential for higher bioavailability of solid oral ITZ through intestinal targeting was demonstrated via pH shift. It was not possible to quantify the molecularly dissolved ITZ through reverse dialysis method, which lacks further development and optimization. The obtained results show that the compendial dissolution methodology is not enough to evaluate poorly-soluble dosage forms performance because they can often lead to a sub or over estimation of its solubility. | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10362/99428 | |
| dc.language.iso | eng | pt_PT |
| dc.subject | in vitro | pt_PT |
| dc.subject | in silico | pt_PT |
| dc.subject | dissolution | pt_PT |
| dc.subject | biorelevant | pt_PT |
| dc.subject | poorly-soluble-drugs | pt_PT |
| dc.subject | free-drug | pt_PT |
| dc.title | In vitro and in silico Dissolution and Permeation Assessment | pt_PT |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | masterThesis | pt_PT |
| thesis.degree.name | Mestre em Engenharia Química e Bioquímica | pt_PT |
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