A carregar...
Publicação
Efeito de um extrato de amora em biomarcadores de esteatose hepática não-alcóolica num modelo de ratinho humanizado com obesidade
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 1.88 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
Resumo Introdução: A esteatose hepática não alcoólica (EHNA) está intimamente associada à obesidade. Apesar do consumo de frutos vermelhos ricos em antocianinas, nomeadamente a amora, ter um efeito protetor no desenvolvimento e progressão da EHNA, e da obesidade a ela associada, os mecanismos moleculares subjacentes encontram-se ainda por explorar. Objetivo: Avaliar o impacto do consumo de um extrato de amora nos biomarcadores metabólicos de EHNA, num modelo de ratinho humanizado com obesidade induzida por disbiose e dieta rica em lípidos e sacarose. Material e métodos: Ratinhos machos C57BL/6J foram aleatoriamente distribuídos em 3 grupos (n=8 ratinhos/grupo): 1) grupo DH, ratinhos com obesidade induzida por dieta rica em lípidos e sacarose durante 5 semanas; 2) grupo DH+TMF, ratinhos com obesidade induzida simultaneamente por transplante fecal de microbiota proveniente de dadores obesos e dieta rica em lípidos e sacarose; e 3) grupo DH+TMF+AA, ratinhos com obesidade induzida simultaneamente por transplante de microbiota e dieta rica em lípidos e sacarose, aos quais foi administrado um extrato de amora. A concentração hepática e sérica de triacilgliceróis e glicerol livre, e a expressão hepática de proteínas chave da via de sinalização da insulina (Akt) e da síntese de ácidos gordos (carboxílase da acetil-coA), foram determinadas. Resultados: A concentração de triacilgliceróis e a expressão proteica da Akt, ambas a nível hepático, foram semelhantes nos 3 grupos. Contudo, a concentração plasmática de trialcilgliceróis diminuiu cerca de 25-35% no grupo DH+TMF+AA comparativamente aos grupos DH+TMF e DH, estando este efeito associado a um aumento, da mesma ordem de grandeza, da concentração plasmática de glicerol livre no grupo DH+TMF+AA. Relativamente à expressão hepática da carboxílase da acetil-coA, verificou-se um aumento, superior a 50%, no grupo DH+TMF+AA comparativamente aos grupos DH+TMF e DH. Conclusão: A nível hepático, o extrato de amora não altera nem a acumulação de triacilgliceróis nem a expressão de proteínas chave da via de sinalização da insulina em ratinhos humanizados com obesidade. Contudo, o extrato de amora é capaz de reduzir a concentração circulante de triacilgliceróis, levando a um aumento, eventualmente compensatório, da lipogénese hepática.
Abstract: Background: Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity. Although the consumption of anthocyanin-rich fruits, namely blackberry, has a protective effect on the development and progression of NAFLD, and its related obesity, the underlying molecular mechanisms are yet to be explored. Aim: To evaluate the impact of the consumption of a blackberry extract on the metabolic biomarkers of NAFLD, in a humanized mouse model of obesity induced by dysbiosis and a diet rich in lipids and sugar. Material and methods: C57BL/6J male mice were randomly distributed into 3 groups (n=8 mice/group): 1) DH group, mice with obesity induced by a diet rich in lipids and sugar for 5 weeks; 2) DH+TMF group, mice with obesity induced simultaneously by fecal microbiota transplantation from obese donors and a diet rich in lipids and sugar; and 3) DH+TMF+AA group, mice with obesity induced simultaneously by microbiota transplantation and a diet rich in lipids and sugar, and administered with a blackberry extract. Hepatic and serum concentrations of triacylglycerols and free glycerol, and the hepatic expression of key proteins involved in the insulin signaling pathway (Akt) and fatty acid synthesis (acetyl-coA carboxylase), were determined. Results: The concentration of triacylglycerols and the protein expression of Akt, both at hepatic level, were similar in the 3 groups. However, plasma triacylglycerols concentration decreased by about 25-35% in the DH+TMF+AA group compared to both DH+TMF and DH groups, being effect accompanied an increase, of similar magnitude, in the plasma concentration of free glycerol in the DH+TMF+AA group. Regarding the hepatic expression of acetyl-coA carboxylase, there was an increase of more than 50% in the DH+TMF+AA group compared to DH+TMF and DH groups. Conclusion: At hepatic level, blackberry extract does not alter either the accumulation of triacylglycerols or the expression of key proteins of the insulin signaling pathway in humanized obese mice. However, blackberry extract is capable of reducing the circulating concentration of triacylglycerols, leading to a potential increase in hepatic lipogenesis.
Abstract: Background: Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity. Although the consumption of anthocyanin-rich fruits, namely blackberry, has a protective effect on the development and progression of NAFLD, and its related obesity, the underlying molecular mechanisms are yet to be explored. Aim: To evaluate the impact of the consumption of a blackberry extract on the metabolic biomarkers of NAFLD, in a humanized mouse model of obesity induced by dysbiosis and a diet rich in lipids and sugar. Material and methods: C57BL/6J male mice were randomly distributed into 3 groups (n=8 mice/group): 1) DH group, mice with obesity induced by a diet rich in lipids and sugar for 5 weeks; 2) DH+TMF group, mice with obesity induced simultaneously by fecal microbiota transplantation from obese donors and a diet rich in lipids and sugar; and 3) DH+TMF+AA group, mice with obesity induced simultaneously by microbiota transplantation and a diet rich in lipids and sugar, and administered with a blackberry extract. Hepatic and serum concentrations of triacylglycerols and free glycerol, and the hepatic expression of key proteins involved in the insulin signaling pathway (Akt) and fatty acid synthesis (acetyl-coA carboxylase), were determined. Results: The concentration of triacylglycerols and the protein expression of Akt, both at hepatic level, were similar in the 3 groups. However, plasma triacylglycerols concentration decreased by about 25-35% in the DH+TMF+AA group compared to both DH+TMF and DH groups, being effect accompanied an increase, of similar magnitude, in the plasma concentration of free glycerol in the DH+TMF+AA group. Regarding the hepatic expression of acetyl-coA carboxylase, there was an increase of more than 50% in the DH+TMF+AA group compared to DH+TMF and DH groups. Conclusion: At hepatic level, blackberry extract does not alter either the accumulation of triacylglycerols or the expression of key proteins of the insulin signaling pathway in humanized obese mice. However, blackberry extract is capable of reducing the circulating concentration of triacylglycerols, leading to a potential increase in hepatic lipogenesis.
Descrição
Palavras-chave
Non-alcoholic fatty liver disease Obesity Microbiota Blackberry