ITQB: Master Thesis-PMI
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- Seedborne microbiota from tolerant rice genotype: a new approach to enhance salt-tolerance in sensitive varietiesPublication . Rebelo-Romão, Inês; Vilchez, Juan; Santos, Ana Paula"Rice is an essential crop worldwide, but it is highly susceptible to abiotic stress conditions, including soil salinity, which is a major problem for agriculture. Plant growth-promoting bacteria (PGPB) can promote plant vigour and adaptation in stressful conditions by producing phytohormones and other compounds. Identifying salt-tolerant PGPB can lead to novel mechanisms for coping with salt stress and providing sustainable solutions for agriculture. Our work aimed to study the effect of ST-PGPB candidates isolated from the seed-borne and endosphere of salt-tolerant rice plants (Pokkali) and their impact on the level of salt stress resistance and responses of the salt-sensitive varieties (Nipponbare and IR-29). We were particularly interested in the seed-borne because the mother plants transfer the bacteria to the next generation in order to provide better adaptability. Initially, the culturable seed-borne and root endophytic microbiota of all cultivars was isolated and genetically identified to evaluate population patterns. Then, a total of eight strains were selected and screened by their skills and growth rate in salt, at different concentrations (up to 2 M), including biofilm formation, auxins and ACC deaminase production. Four of the eight tested strains were selected (Bacillus altitudinis, Bacillus subtilis, Lisinibacillus fusiformis and Paenibacillus pabuli).(...)"