Development of tools for genetic manipulation of Staphylococcus aureus

Abstract

"With the global distribution of multidrug resistant Staphyloccocus aureus, a human pathogen responsible for thousands of deaths every year, there is an increasing need to study its mechanisms of resistance as well as discover new antibiotics that effectively interfere with its growth and division. However, despite the growing interest in studying this bacteria, there are only a few tools currently available for the genetic manipulation of S. aureus. In this study we developed genetic tools that could facilitate DNA insertion and gene repression in S. aureus: pKILL plasmid and CRISPRi.(...)"