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Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
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This thesis is organized in three chapters. Chapter 1 comprises a brief introduction on the methodology used throughout the work herein presented. Chapter 2 describes the production of membrane histidine kinases from Staphylococcus aureus and Clostridium difficile and chapter 3 the structural studies of a thiosulfate dehydrogenase from Campylobacter jejuni. The experimental work was performed in the Membrane Protein Crystallography Laboratory at Instituto de Tecnologia Química e Biológica, and also included a visit to SOLEIL synchrotron, Paris, France, to collect X-ray diffraction data.
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Histidine kinases membrane protein production and purification X-ray crystallography thiosulfate dehydrogenase