ITQB: BCB - MA Dissertations
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- Construction of a promoter library to study Staphylococcus aureus response to antibiotic exposurePublication . Gomes dos Reis ,Letícia; Reed, Patricia"Staphylococcus aureus exposure to antibiotics and the organism’s ability to acquire new DNA has led to the development of Methicillin Resistant Staphylococcus aureus (MRSA) strains. S. aureus has developed resistance to virtually all known classes of antibiotics, therefore there is a need for the development of new therapies and further studies to better understand the Mode of Action (MOA) of new antibiotics. To contribute towards this goal, we aimed to construct a library of promoters and to study their response to the presence of a range of different antibiotics. To achieve this, S. aureus genomic DNA fragments were cloned into a plasmid vector, upstream of the gene encoding the fluorescent mNeonGreen protein. Strains containing the resulting transcriptional fusions were exposed to different antibiotics at varying concentrations.(...)"
- Development of tools for genetic manipulation of Staphylococcus aureusPublication . Serra, Lúcia Branco; Pinho, Mariana"With the global distribution of multidrug resistant Staphyloccocus aureus, a human pathogen responsible for thousands of deaths every year, there is an increasing need to study its mechanisms of resistance as well as discover new antibiotics that effectively interfere with its growth and division. However, despite the growing interest in studying this bacteria, there are only a few tools currently available for the genetic manipulation of S. aureus. In this study we developed genetic tools that could facilitate DNA insertion and gene repression in S. aureus: pKILL plasmid and CRISPRi.(...)"