ITQB: MPC - MA Dissertations
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- Structural studies on enzymes involved in microbial hydrogen sulfide metabolismPublication . Soares Costa, Sofia; Brito, José; Vicente, João"Hydrogen sulfide (H2S) is an ancient molecule present since Earth’s primordial atmosphere. Organisms from all Domains of Life early evolved to use this molecule in their physiologic activities. H2S was first recognized as a potent respiratory toxin, however, recent studies revealed its involvement in processes such as neurotransmission, signaling and inflammation. Therefore, a tight regulation of H2S intracellular concentration is paramount in all organisms.(...)"
- Crystallographic studies of serine β-lactamase and serine proteases with novel inhibitorsPublication . Mamede, Paulo Alexandre Correia; Archer, Margarida"Serine-based enzymes are present in many biological processes and are often chosen as promising drug targets, since their deregulation lead to diseases, and study the 3D structure is essential to understand how they interact with molecules capable of inhibiting their activity. This work aims for structure determination by X-ray crystallography of protein complexes with novel inhibitors and results from a collaboration established with Prof. Rui Moreira from iMed.ULisboa, who provided the ligands.(...)"
- Structural and functional studies on enzymes involved in microbial hydrogen sulfide metabolismPublication . Almeida Cordeiro, Rafaela; Brito, José; Vicente, João"Hydrogen sulfide has a physiological role in the human organism. The tight control and balance of its metabolism is very important since high concentrations of bacterial-derived H2S confers them increased resistance to host leukocytes. Infections caused by Staphylococcus (S.) aureus are very common at present and are associated with resistant strains. S. aureus has a cst operon that encodes proteins responsible for maintaining the tight balance in H2S metab-olism. One of these proteins is SQR, responsible for the first step of H2S detoxification. In collaboration with Giedroc's lab, the major aim of this project is to biochemically and structurally characterize the SQR protein from S. aureus (SaSQR).(...)"
- Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenasePublication . Kulakova, Alina Vitaliyivna; Archer, Margarida; Rosário, Ana Lúcia; Brito, JoséThis thesis is organized in three chapters. Chapter 1 comprises a brief introduction on the methodology used throughout the work herein presented. Chapter 2 describes the production of membrane histidine kinases from Staphylococcus aureus and Clostridium difficile and chapter 3 the structural studies of a thiosulfate dehydrogenase from Campylobacter jejuni. The experimental work was performed in the Membrane Protein Crystallography Laboratory at Instituto de Tecnologia Química e Biológica, and also included a visit to SOLEIL synchrotron, Paris, France, to collect X-ray diffraction data.