ITQB: GSL - PhD Theses
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- Stress response mechanisms underlying metal detoxification in yeastPublication . Caetano, Soraia C. Marques; Rodrigues-Pousada, Claudina; Pimentel, Catarina" The Genomics and Stress Laboratory, at ITQB, headed by Prof. Claudina Rodrigues- Pousada, has been focused on the identification and characterization of stress-responsive genetic programs regulating metal toxicity in the yeast Saccharomyces cerevisiae(...)"
- Yeast Response to Arsenic’s ThreatsPublication . Ferreira, Ana Rita Tomé; Pousada, Claudina Rodrigues-; Menezes, ReginaHuman exposure to elevated levels of inorganic arsenic (As) poses the greatest threat to global public health. However, arsenic trioxide (ATO) is presently the most active antineoplastic agent in the treatment of acute promyelocytic leukaemia (APL) (Trisenox®, Teva Pharm). Despite the success of this drug, the actual mechanism of action is complex and is not completely understood. We have therefore investigated the mechanisms by which the cell responds to arsenic that perturb homeostasis, and how homeostasis can subsequently be restored, while also focusing on the mechanistic understanding of individual protein regulation important for the cell to thwart the cytotoxic effects of arsenic.(...)
- On the road of sequencing the genomes: Past, Present and FuturePublication . Flores, Alcino Orfeu; Pousada, Claudina RodriguesDesulfovibrio gigas is a model organism of sulfate-reducing bacteria of which energy metabolism and stress response have been extensively studied. The complete genomic context of this organism was however not yet available. This thesis is about the sequencing of the D. gigas genome and provides insights into the integrated network of energy conserving complexes and structures present in this bacterium. The work presented describes the technological processes used to sequence the genome. Chapter I is an overview of its most recent advances in bacterial genome sequencing, since the first genome published of Haemophilus influenzae till present; Chapter II is mostly an overview of the 4 different technologies that were necessary to finalize this genome sequencing project: shotgun sequencing through Sanger method and massive DNA sequencing from 3 different next generation sequencing (NGS) platforms: 454 (Roche);(...)
- Role of Yap8 and Yap1 b-ZIP transcription factors in arsenic stressPublication . Amaral, Catarina Sá Almeida; Rodrigues-Pousada, ClaudinaArsenic compounds are highly toxic substances; nevertheless they are used in the treatment of acute promyelocytic leukaemia. Therefore it is pressing to gain knowledge on its toxicity and detoxification mechanisms. The cellular entry pathways have been discovered and by transcriptome analysis it is known that arsenic activates the transcription of genes activated by, among others, Rpn4, Met4 and Yap1.(...)
- Deciphering the genome of Desulfovibrio gigas: the role of the hydrogenasesPublication . Morais e Silva, Fábio; Pousada, Claudina Rodrigues-The work presented in this thesis describes the functional characterization of hydrogenases in the overall energy metabolism of the sulfate reducing bacterium Desulfovibrio gigas. With the complete annotation of the D. gigas genome, we were able to verify that only the two previously described hydrogenases are present in this organism, the periplasmic [NiFe] HynAB and the cytoplasmic membrane-bound [NiFe] Ech.(...)
- Yeast as a model system to study genetic and post-translational regulation of metabolic pathways in mammalsPublication . Batista-Nascimento, Liliana; Rodrigues-Pousada, ClaudinaThe work presented in this thesis describes the use of yeast Saccharomyces cerevisiae as a model system to study two different stress response processes and its extrapolation to higher eukaryotes.(...)
- Deciphering the role of Yap4 phosphorylation under stress conditionsPublication . Pereira, Jorge; Rodrigues-Pousada, ClaudinaThe existence of molecular mechanisms of response, repair and adaptation, many of which are greatly conserved across nature, gives to the cell with the plasticity it requires to adjust to its ever-changing environment, a homeostatic event that is termed the stress response. In the budding yeast Saccharomyces cerevisiae there is a particular family of transcription factors, the Yap family, which has been shown to have a relevant role in yeast adaptation to several stress conditions. In particular, Yap1 is the major regulator of the transcriptional response to oxidative stress and Yap2 and Yap8 play important roles upon cadmium and arsenic exposure, respectively.(...)