ITQB: BET - MA Dissertations
URI permanente para esta coleção:
Navegar
Entradas recentes
- Exploring malate:quinone oxidoreductases – MQOPublication . Pires, Tatiana Clemente; Pereira, Manuela"Malate:quinone oxidoreductases (MQOs) are membrane-bound proteins that catalyze the oxidation of malate to oxaloacetate and the reduction of the quinone to quinol. MQOs are considered proteins with a central role in the metabolism of the organisms, because they include the Tricarboxylic Acid Cycle, the respiratory chain and in some cases MQOs can also be part of other pathways, like the fumarate cycle.(...)"
- Expression and purification of proteins involved in the mitochondrial electron transport chainPublication . Silva, José Pedro Malanho da; Pereira, Manuela; Duarte, AfonsoNADH: ubiquinone oxidoredutase is one of the most intricate enzymes. It is part of the respiratory chains and is present in several organisms. This enzyme couples the transfer of two electron, from NADH to ubiquinone, and translocate ions across the membrane establishing a transmembrane difference of electrochemical potential. In this work, we present the initial experimental steps to express and purify a subunit from this intricate enzyme, which is directly related with the translocation of ions (i.e. NuoL). The objective was to characterize the subunit reconstituted in membrane-mimicking nanoparticles called nanodiscs using solution Nuclear Magnetic Resonance spectroscopy.
- Structural and functional investigation of type II NADH:quinone oxidoreductase from the pathogenic bacteria Vibrio cholerae and Staphylococcus aureusPublication . Sousa, Filipe Rodrigues Medina de; Pereira, Manuela; Batista, Ana PaulaStaphylococcus aureus and Vibrio cholerae are two pathogenic bacteria that, on their own, are responsible for more than 150,000 human deaths per year (affecting almost 5,000,000 people worldwide). Antibiotics and vaccines have been proved to work as a viable strategy to treat bacterial related epidemics such as cholera and MRSA associated infections. Nevertheless, new drugs are increasingly necessary due to the appearance of resistant strains.