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Ellipsometry and differential interference contrast microscopic imaging of cellular exoand endocytosis: modelling and experiments
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In this work it is presented a solution to Maxwell’s equations for core-shell nanoparticle
scattering near an isotropic substrate covered with an anisotropic thin film, based on
an extension of the Bobbert-Vlieger (BV) solution for particle scattering near a substrate,
delivering an exact solution in the near-field as well as far-field. It is applied successfully
the developed scattering model to the calculation of light scattering on an optical model
representing a lipid vesicle near a lipid bilayer, whereby the lipids are characterized
through a uniaxial optical model. Hereby, it is paved the path for understanding quantitatively
how light scatters during a cellular exo- or endocytosis event during microscopic
observation taking into account lipid induced anisotropy. Through the application of ellipsometry
angles it is effectively demonstrated that realistically small optical anisotropy
values significantly alter far-field optical scattering in respect to an equivalent optical
model for cellular endocytosis consisting of isotropic components only.
It is then calculated the impact of lipid-induced optical anisotropy on the experimental
observation of exo- or endocytic microscopic imaging with e.g. Differential Interference
Contrast (DIC) microscopy. Furthermore, it is integrated this extended BV scattering
solution into a rigorous model of DIC image formation which allows for characterizing
DIC, through simulation, as a tool for imaging of exo- or endocytosis events. It is also
compared theoretical predictions with experimental high Numerical Aperture (NA) dic
imaging of dielectric oxide nanoparticles with organic shell.
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Palavras-chave
Optical properties of nanostructures Optical anisotropy Nanomedicine