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Microalgae for the Production of High-Value Compounds: Evaluation of the Effect of Different Nitrogen and Carbon Sources on the Production of Fucoxanthin in Phaeodactylum tricornutum
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Autores
Bragança, Ana Rita
Orientador(es)
Resumo(s)
"Phaeodactylum tricornutum is a model diatom used in studies of growth and production of
photosynthetic pigments, with the particularity of its great capacity to produce fucoxanthin. Fucoxanthin
is a high-value pigment with applications in the food, cosmetic and pharmaceutical industries, revealing
in pre-clinical studies, antioxidant, anti-obesity, and anti-cancer activity. This xanthophyllous pigment
plays an important role in microalgae, as its production occurs in response to abiotic stresses, allowing
the cell to survive under less favourable conditions. Thus, it is possible to optimize the production of
fucoxanthin by changing the growth conditions, in particular the composition of the culture medium.
In this thesis, changes in the culture medium composition were studied, through the modification
of nitrogen and carbon sources in various concentrations. The results indicated that the conditions that
most favoured the growth and production of fucoxanthin were achieved by increasing sodium nitrate by
a factor of 10 to the f/2 medium supplemented with silica, which is used for maintenance of the diatoms
growth. The increase in the concentration of sodium nitrate allowed to double the fucoxanthin
production, in just 7 days of growth. Cell viability and behaviour under different light conditions were
also investigated. Additionally, genetic expression studies were performed to evaluate the possible
influence of N sources on the expression of target genes of the fucoxanthin synthesis pathway.(...)"
Descrição
Palavras-chave
Phaeodactylum tricornutum Photoperiod Cycle Cell Viability Carotenoid Biosynthetic Pathway Nitrogen Sources Fucoxanthin