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Publicação
Live cell biosensors for detection and quantification of viral pathogens
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| 4.78 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
"Every year, thousands of human lives are lost due to viral pathogen infections, namely Zika and
Chikungunya. Currently, no vaccines or antivirals are available to prevent and treat these infections.
This current and imperative medical necessity is detained by the urgent need for reliable tools to study
these viruses in both fundamental and applied research.
To tackle the presented problems, we developed, (i) a platform relying on a cell-based approach
for viral detection and (ii) a virus-based tool for antiviral screenings. For the cell-based approach, split-
GFP with prevented transcomplementation by single – embedment in an eglin c molecule (eGFP11) or
intein cyclization (cyGFP11) – or dual distortions – using coiled-coils (ccGFP10+ccGFP11) – was used.(...)"
Descrição
Palavras-chave
Zika antiviral screenings Chikungunya chimeric retrovirus-like particles Cell-based biosensor protease virus detection
Contexto Educativo
Citação
Editora
Instituto de Tecnologia Química e Biológia António Xavier. Universidade NOVA de Lisboa.