Validation and Optimization of a Structure-Based Enzyme Engineering Platform

Utilize este identificador para referenciar este registo: http://hdl.handle.net/10362/181407

Título:	Validation and Optimization of a Structure-Based Enzyme Engineering Platform
Autor:	João Pedro, Gorjão Araújo
Orientador:	Soares, Cláudio Lousa, Diana
Palavras-chave:	Enzyme engineering Machine learning Scoringfunctions Biosyntheticpathways Automatedplatform
Data de Defesa:	7-Mar-2024
Resumo:	"Organismsandenzymesarecurrentlyusedforcatalyzingtheproductionofvalue- added compounds,indiverseareaswitheconomicimpact.However,poorprotein expression, lowcatalyticlevels,lowconcentrationsofco-factorsorsubstrates,toxicity towardsthefinalproductandotherfactors,allcontributetothelowperformanceof microorganismsandenzymesintheseareas.Proteinengineeringisaneffectivestrategy forimprovingbiosyntheticpathwaysandovercomingtheseproblems,byre-designingan enzyme’scatalyticpropertiesinfavorofacertainreaction. Toaddressthisproblem,theProteinModelingLaboratoryofITQBNOVAdeveloped an automatedplatformforgenediscoveryandenzymeengineering,whichaimstoenhance or changetheenzymesthatcatalyzethelimitingstepsinpathwaysthatcanbeexploited fortheproductionofaddedvaluecompounds.Theobjectivesrangedfromincreasing an enzyme’sefficiencytoenablingittocatalyzeadifferenttransformationthantheone catalyzedbythewild-typesequence.(...)"
URI:	http://hdl.handle.net/10362/181407
Designação:	Dissertation presented to obtain the Master degree in Computacional Biology and Bioinformatics
Aparece nas colecções:	ITQB: PM - MA Dissertations

Ficheiros deste registo:

Ficheiro	Descrição	Tamanho	Formato
Master_dissertation_João_Correia.pdf		8,85 MB	Adobe PDF	Ver/Abrir Acesso Restrito. Solicitar cópia ao autor!