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Validation and Optimization of a Structure-Based Enzyme Engineering Platform
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 8.64 MB | Adobe PDF |
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"Organismsandenzymesarecurrentlyusedforcatalyzingtheproductionofvalue-
added compounds,indiverseareaswitheconomicimpact.However,poorprotein
expression, lowcatalyticlevels,lowconcentrationsofco-factorsorsubstrates,toxicity
towardsthefinalproductandotherfactors,allcontributetothelowperformanceof
microorganismsandenzymesintheseareas.Proteinengineeringisaneffectivestrategy
forimprovingbiosyntheticpathwaysandovercomingtheseproblems,byre-designingan
enzyme’scatalyticpropertiesinfavorofacertainreaction.
Toaddressthisproblem,theProteinModelingLaboratoryofITQBNOVAdeveloped
an automatedplatformforgenediscoveryandenzymeengineering,whichaimstoenhance
or changetheenzymesthatcatalyzethelimitingstepsinpathwaysthatcanbeexploited
fortheproductionofaddedvaluecompounds.Theobjectivesrangedfromincreasing
an enzyme’sefficiencytoenablingittocatalyzeadifferenttransformationthantheone
catalyzedbythewild-typesequence.(...)"
Descrição
Palavras-chave
Enzyme engineering Machine learning Scoringfunctions Biosyntheticpathways Automatedplatform