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Insight into the biophysical properties of AsP2Ox, a candidate enzyme for health diagnosis biosensors
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"Pyranose-2-oxidases (P2Oxs) are flavoenzymes capable of oxidizing a wide range of sugars, in particular D-glucose, with concomitant reduction of molecular oxygen to hydrogen peroxide. The vast majority of P2Oxs were identified in fungi, but recently, bacterial P2Oxs were characterized showing distinctive and interesting properties to be explored in biotechnology. This work aims to unveil structural details of the bacterial P2Ox from Pseudoarthrobacter siccitolerans (AsP2Ox). Protein crystals were obtained in space group C2221 space group and diffracted X-rays to a resolution of 2.01 Å, and a crystal structure of the AsP2Ox-Glucose complex at 2.35 Å resolution was also obtained. The typical P2Oxs substrate and FAD-binding domains were identified, and a flexible loop (substrate loop) found in fungal P2Oxs with a role of gating and accommodating the substrate in the active site is also present in AsP2Ox.(...)"
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Pyranose 2-oxidases structural details flavoenzymes biotechnology biosensors