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Unveiling the OsPIF15 and OsPIF16 regulatory network underlying rice coleoptile growth
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Orientador(es)
Resumo(s)
"Light is a key environmental cue, influencing most stages of plant development and growth, including
seedling establishment. Upon seedling emergence, red/far-red light-absorbing phytochromes (phys) are
light-activated and translocated into the nucleus, where they interact with phytochrome-interacting
factors [PIFs; bHLH transcription factors (TFs)], promoting PIF degradation or inhibiting PIFs from
binding to their DNA targets, and shifting the seedling’s developmental program from skoto- to
photomorphogenesis. In rice, three phytochromes (OsphyA-C) and seven PIFs (OsPIF11-16, OsPIF8)
have been identified, but this regulatory network remains fairly unknown. When CRISPR/Cas9 knockout
single and double ospif mutants were grown under dark, ospif15 and ospif16 seedlings developed
shorter coleoptiles than the wild-type (WT), but the difference was greater in the ospif15 ospif16 lines.
Under white, red and far-red light, there were no significant differences in coleoptile length between
mutants and the WT. Thus, OsPIF15 and OsPIF16 were identified as positive regulators of
skotomorphogenesis. To further uncover these TF’s functional roles in light-dependent regulation of
coleoptile growth, we investigated their interactions with OsphyA-C. Both OsPIFs interacted with
OsphyB, but only OsPIF15 interacted with OsphyA. As members of the bHLH TF family, their ability to
homo- and heterodimerize was tested as well, and preliminary results showed that both OsPIF15 and
OsPIF16 form homo- and hetero-dimers. OsphyB-mediated degradation of OsPIF15 by light has already
been described. We analyzed OsPIF16 stability under light in WT and phyB-1 mutants and showed that
OsPIF16 degradation by light is OsphyB-dependent(...)"
Descrição
Palavras-chave
phytochrome-interacting factor (PIF) coleoptile elongation skotomorphogenesis light signaling
Contexto Educativo
Citação
Editora
Instituto de Tecnologia Química e Biológica António Xavier. Universidade NOVA de Lisboa