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Publicação
Toward a role for LOTUS domain containing proteins during sexual reproduction in Arabidopsis thaliana
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| 9.88 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
"Plant reproduction study is key to increase crop yield and resilience to climate change. The LOTUS
domain comprises ~100 amino acid and structurally form a winged helix-turn-helix. In animal, LOTUS
domain containing proteins (LDCPs) have been associated with oogenesis and spermatogenesis but
their role in plant remains elusive. The goal of this project is to understand the role of LDCPs in the
reproductive process of Arabidopsis thaliana. Data acquired from gene expression suggested that gene
AT2G05160 (LDCP1) and AT3G52980 (LDCP2) are specifically and highly expressed in reproductive
tissues. Previously published results indicate LDCP2 to be important for pollen development. The role
of LDCP1 and LDCP2 was investigated through reverse genetic approaches using T-DNA lines and
CRISPR-Cas9 technology to obtain mutants, including a double mutant.(...)"
Descrição
Palavras-chave
Arabidopsis LOTUS Domain T-DNA mutant CRISPR-Cas9
Contexto Educativo
Citação
Editora
Instituto de Tecnologia Química e Biológica António Xavier. Universidade NOVA de Lisboa