A carregar...
Publicação
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 2.92 MB | Adobe PDF |
Orientador(es)
Resumo(s)
The brown seaweed Spatoglossum schröederi synthesizes three bioactive fucoidans, the most abundant of which is fucan A. This fucoidan was extracted and its identity was confirmed by chemical analysis, Fourier-transform infrared spectroscopy (FTIR), and agarose gel electrophoresis. Thereafter, silver nanoparticles containing fucan A (AgFuc) were produced using an environmentally friendly synthesis method. AgFuc synthesis was analyzed via UV–vis spectroscopy and FTIR, which confirmed the presence of both silver and fucan A in the AgFuc product. Dynamic light scattering (DLS), X-ray diffraction, scanning electron microscopy, and atomic force microscopy revealed that the AgFuc particles were ~180.0 nm in size and spherical in shape. DLS further demonstrated that AgFuc was stable for five months. Coupled plasma optical emission spectrometry showed that the AgFuc particles contained 5% silver and 95% sugar. AgFuc was shown to be more effective in inhibiting the ability of parasites to reduce MTT than fucan A or silver, regardless of treatment time. In addition, AgFuc induced the death of ~60% of parasites by necrosis and ~17% by apoptosis. Therefore, AgFuc induces damage to the parasites’ mitochondria, which suggests that it is an anti-Trypanosoma cruzi agent. This is the first study to analyze silver nanoparticles containing fucan as an anti-Trypanosoma cruzi agent. Our data indicate that AgFuc nanoparticles have potential therapeutic applications, which should be determined via preclinical in vitro and in vivo studies.
Descrição
Funding Information: Funding: This research was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (n◦ 408369/2016-7), Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Programa Ciências do Mar (AUXPE-CIMAR-1956/2014), and Programa Nacional de Cooperação Acadêmica (CAPES/PROCAD). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Palavras-chave
anti-Trypanosoma cruzi activity brown seaweed chagas disease fucoidan RA0421 Public health. Hygiene. Preventive Medicine RM Therapeutics. Pharmacology QD Chemistry General Chemical Engineering General Materials Science Parasitology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 7 - Affordable and Clean Energy SDG 11 - Sustainable Cities and Communities