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Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity

2022-06-01Artigo científico Acesso aberto
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dc.contributor.authorSouza, Adriana Oliveira
dc.contributor.authorOliveira, Johny Wysllas de Freitas
dc.contributor.authorMoreno, Claudia Jéssica Gonsalves
dc.contributor.authorde Medeiros, Mayra Jane Campos
dc.contributor.authorFernandes-Negreiros, Marília Medeiros
dc.contributor.authorSouza, Flavia Roberta Monteiro
dc.contributor.authorPontes, Daniel Lima
dc.contributor.authorSilva, Marcelo Sousa
dc.contributor.authorRocha, Hugo Alexandre Oliveira
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.institutionUniversidade NOVA de Lisboa
dc.contributor.pblMDPI AG
dc.date.accessioned2023-02-08T22:22:18Z
dc.date.available2023-02-08T22:22:18Z
dc.date.issued2022-06-01
dc.descriptionFunding Information: Funding: This research was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (n◦ 408369/2016-7), Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Programa Ciências do Mar (AUXPE-CIMAR-1956/2014), and Programa Nacional de Cooperação Acadêmica (CAPES/PROCAD). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractThe brown seaweed Spatoglossum schröederi synthesizes three bioactive fucoidans, the most abundant of which is fucan A. This fucoidan was extracted and its identity was confirmed by chemical analysis, Fourier-transform infrared spectroscopy (FTIR), and agarose gel electrophoresis. Thereafter, silver nanoparticles containing fucan A (AgFuc) were produced using an environmentally friendly synthesis method. AgFuc synthesis was analyzed via UV–vis spectroscopy and FTIR, which confirmed the presence of both silver and fucan A in the AgFuc product. Dynamic light scattering (DLS), X-ray diffraction, scanning electron microscopy, and atomic force microscopy revealed that the AgFuc particles were ~180.0 nm in size and spherical in shape. DLS further demonstrated that AgFuc was stable for five months. Coupled plasma optical emission spectrometry showed that the AgFuc particles contained 5% silver and 95% sugar. AgFuc was shown to be more effective in inhibiting the ability of parasites to reduce MTT than fucan A or silver, regardless of treatment time. In addition, AgFuc induced the death of ~60% of parasites by necrosis and ~17% by apoptosis. Therefore, AgFuc induces damage to the parasites’ mitochondria, which suggests that it is an anti-Trypanosoma cruzi agent. This is the first study to analyze silver nanoparticles containing fucan as an anti-Trypanosoma cruzi agent. Our data indicate that AgFuc nanoparticles have potential therapeutic applications, which should be determined via preclinical in vitro and in vivo studies.en
dc.description.versionpublished
dc.format.extent3059995
dc.identifier.doi10.3390/nano12122059
dc.identifier.issn2079-4991
dc.identifier.otherPURE: 50620770
dc.identifier.otherPURE UUID: 0f2cc039-2adc-403c-b3b0-3182c23fc81f
dc.identifier.otherScopus: 85132116239
dc.identifier.otherPubMed: 35745396
dc.identifier.otherWOS: 000818321300001
dc.identifier.urihttp://hdl.handle.net/10362/148883
dc.identifier.urlhttps://www.scopus.com/pages/publications/85132116239
dc.language.isoeng
dc.peerreviewedyes
dc.subjectanti-Trypanosoma cruzi activity
dc.subjectbrown seaweed
dc.subjectchagas disease
dc.subjectfucoidan
dc.subjectRA0421 Public health. Hygiene. Preventive Medicine
dc.subjectRM Therapeutics. Pharmacology
dc.subjectQD Chemistry
dc.subjectGeneral Chemical Engineering
dc.subjectGeneral Materials Science
dc.subjectParasitology
dc.subjectSDG 3 - Good Health and Well-being
dc.subjectSDG 9 - Industry, Innovation, and Infrastructure
dc.subjectSDG 7 - Affordable and Clean Energy
dc.subjectSDG 11 - Sustainable Cities and Communities
dc.titleSilver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activityen
dc.typejournal article
degois.publication.issue12
degois.publication.titleNanomaterials
degois.publication.volume12
dspace.entity.typePublication
rcaap.rightsopenAccess

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