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Using gold nanobeacons as a theranostic technique to recognize, detect, and inhibit specific nucleic acids
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Orientador(es)
Resumo(s)
This protocol describes the synthesis and characterization of gold nanoparticle-based nanobeacons as a theranostic strategy for the recognition, detection, and inhibition of miRNA and mRNA. This system is designed for an in vitro evaluation of a sequence's silencing potential and later used for cellular and in vivo gene silencing approaches using fluorescence imaging, enhancing theranostic procedures in which nanoparticle-based sensors and inhibitors may provide simultaneous detection of different gene-associated conditions and nanodevices for a real-time monitoring of gene delivery. For complete details on the use and execution of this protocol, please refer to Conde et al. (2015, 2013).1,2.
Descrição
Funding: The authors acknowledge Fundação para a Ciência e a Tecnologia (FCT) for the financial support in the framework of the PhD grant 2020.06638.BD (D.P.S.) and the European Research Council Starting Grant ERC-StG-2019- 848325 (J.C.). Figures 1, 2, 4, and 5 were created with BioRender.com.