Publicação
Using gold nanobeacons as a theranostic technique to recognize, detect, and inhibit specific nucleic acids
| dc.contributor.author | Sousa, Diana P. | |
| dc.contributor.author | P. Sousa, Diana | |
| dc.contributor.author | Conde, João | |
| dc.contributor.author | Conde, João | |
| dc.contributor.institution | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) | |
| dc.contributor.institution | Centre for Toxicogenomics and Human Health (ToxOmics) | |
| dc.date.accessioned | 2022-12-27T22:16:21Z | |
| dc.date.available | 2022-12-27T22:16:21Z | |
| dc.date.issued | 2022-12-16 | |
| dc.description | Funding: The authors acknowledge Fundação para a Ciência e a Tecnologia (FCT) for the financial support in the framework of the PhD grant 2020.06638.BD (D.P.S.) and the European Research Council Starting Grant ERC-StG-2019- 848325 (J.C.). Figures 1, 2, 4, and 5 were created with BioRender.com. | |
| dc.description.abstract | This protocol describes the synthesis and characterization of gold nanoparticle-based nanobeacons as a theranostic strategy for the recognition, detection, and inhibition of miRNA and mRNA. This system is designed for an in vitro evaluation of a sequence's silencing potential and later used for cellular and in vivo gene silencing approaches using fluorescence imaging, enhancing theranostic procedures in which nanoparticle-based sensors and inhibitors may provide simultaneous detection of different gene-associated conditions and nanodevices for a real-time monitoring of gene delivery. For complete details on the use and execution of this protocol, please refer to Conde et al. (2015, 2013).1,2. | en |
| dc.description.version | publishersversion | |
| dc.description.version | published | |
| dc.format.extent | 5703586 | |
| dc.identifier.doi | 10.1016/j.xpro.2022.101916 | |
| dc.identifier.other | PURE: 48425162 | |
| dc.identifier.other | PURE UUID: 4d06d602-41ef-4b25-9f27-1b317610a37d | |
| dc.identifier.other | PubMedCentral: PMC9758562 | |
| dc.identifier.other | Scopus: 85145430822 | |
| dc.identifier.other | PubMed: 36595910 | |
| dc.identifier.uri | http://hdl.handle.net/10362/146695 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.title | Using gold nanobeacons as a theranostic technique to recognize, detect, and inhibit specific nucleic acids | en |
| dc.type | journal article | |
| degois.publication.issue | 4 | |
| degois.publication.title | STAR Protocols | |
| degois.publication.volume | 3 | |
| dspace.entity.type | Publication | |
| person.familyName | P. Sousa | |
| person.familyName | Conde | |
| person.givenName | Diana | |
| person.givenName | João | |
| person.identifier | 807432 | |
| person.identifier.ciencia-id | A71C-B10E-255E | |
| person.identifier.orcid | 0000-0003-3474-5417 | |
| person.identifier.orcid | 0000-0001-8422-6792 | |
| person.identifier.rid | F-2231-2011 | |
| person.identifier.scopus-author-id | 56992468300 | |
| rcaap.rights | openAccess | |
| relation.isAuthorOfPublication | d45152bc-77a6-41db-8b9c-f0f907726aad | |
| relation.isAuthorOfPublication | 33ca4178-1b25-4b5e-ae16-b2500abe4c58 | |
| relation.isAuthorOfPublication.latestForDiscovery | 33ca4178-1b25-4b5e-ae16-b2500abe4c58 |
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