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Produção de estruturas 3D porosas à base de polímeros piezoelétricos para aplicação em engenharia de tecidos
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Os biomateriais são projetados para reproduzir a matriz extracelular e atuam como suporte físico que promove o crescimento do tecido e a migração das proteínas. Esta área está em desenvolvimento devido à crescente necessidade de transplantes e problemas relacionados com a biocompatibilidade.
O Fluoreto de polivinilideno (PVDF) é um fluoropolímero e um biopolímero. As membranas de PVDF foram produzidas e avaliadas como membranas para as células. No entanto, estas membranas não fizeram proveito da fase β do PVDF, que exibe propriedades piezoeléctricas. Devido ao papel que a resposta piezoeléctrica na remodelação do osso, as membranas piezoeléctricas podem ser aproveitadas para aumentar a eficácia de reparação do tecido ósseo. Neste trabalho foram produzidas membranas de PVDF pelos métodos de solvent-casting and particulate leaching e CO2 supercrítico de forma a se obter uma membrana com poros interconectados.
As membranas otimizadas de PVDF foram obtidas apenas pelo método do CO2 supercrítico e não por outro tipo de método. As membranas produzidas puderam confirmar a presença de fase β. As imagens obtidas por DRX e SEM evidenciaram uma estrutura interconectada altamente porosa. A porosidade da estrutura, os testes de adesão celular, a polarização elétrica e os ensaios de bioactividade também foram estudados.
Biomaterial scaffolds are designed to mimic the extracellular matrix and will act as a physical support to promote the regrowth of the tissue and the migration of proteins. This field is still in development because of the emerging need of transplants and its biocompatibility issues. Polyvinylidene fluoride (PVDF) is a fluoropolymer and biopolymer. PVDF membranes have already been produced and evaluated as scaffolds. However, these membranes did not make use of the β-phase of PVDF, that exhibits piezoelectric properties. Because of the role played by piezoelectric response on bone remodelling, piezoelectric scaffolds can be exploited to enhance the efficacy of repair in the bone tissue. In this work PVDF membranes were produced by solvent-casting and particulate leaching and CO2 supercritical methods in order to get a membrane with interconnected porosity. Optimised PVDF membranes by CO2 supercritical were obtained but not by other method. The membranes showed the presence of β-phase. DRX and SEM images displayed a highly interconnected porous structure. The porosity of the structure, cell adhesion, biocompatibility, electrical polarization and bioactivity were also studied in this work.
Biomaterial scaffolds are designed to mimic the extracellular matrix and will act as a physical support to promote the regrowth of the tissue and the migration of proteins. This field is still in development because of the emerging need of transplants and its biocompatibility issues. Polyvinylidene fluoride (PVDF) is a fluoropolymer and biopolymer. PVDF membranes have already been produced and evaluated as scaffolds. However, these membranes did not make use of the β-phase of PVDF, that exhibits piezoelectric properties. Because of the role played by piezoelectric response on bone remodelling, piezoelectric scaffolds can be exploited to enhance the efficacy of repair in the bone tissue. In this work PVDF membranes were produced by solvent-casting and particulate leaching and CO2 supercritical methods in order to get a membrane with interconnected porosity. Optimised PVDF membranes by CO2 supercritical were obtained but not by other method. The membranes showed the presence of β-phase. DRX and SEM images displayed a highly interconnected porous structure. The porosity of the structure, cell adhesion, biocompatibility, electrical polarization and bioactivity were also studied in this work.
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biomaterial PVDF propriedades piezoeléctricas fase β adesão celular