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Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells
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Orientador(es)
Resumo(s)
Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand. © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro.
Descrição
Authors thank Xunta de Galicia (Biomedicine) for the research grant 10CSA383009PR, and Scientific PROTEOMASS Association (Portugal) for financial support. Elisabete Oliveira and Hugo M. Santos thank to MEC-FCT (Portugal), for the post-doctoral grants SRFH/BPD/72557/2010 and SRFH/BPD/73997/2010. Julia Lorenzo thanks to Ministerio de Ciencia e Innovacion (MICINN) of Spain, for grant BIO2010-22321-C02. Authors thank the financial support by REQUIMTE-FCT PEst-C/EQB/LA0006/2013.
Palavras-chave
Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles