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Isolation, identification and biotechnological applications of a novel, robust, free-living chlorococcum (Oophila) amblystomatis strain isolated from a local pond

2020-04-27Artigo científico Acesso aberto
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dc.contributor.authorCorreia, Nádia
dc.contributor.authorPereira, Hugo
dc.contributor.authorSilva, Joana T.
dc.contributor.authorSantos, Tamára
dc.contributor.authorSoares, Maria
dc.contributor.authorSousa, Carolina B.
dc.contributor.authorSchüler, Lisa M.
dc.contributor.authorCosta, Margarida
dc.contributor.authorVarela, João
dc.contributor.authorPereira, Leonel
dc.contributor.authorSilva, Joana
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2025-03-17T21:09:56Z
dc.date.available2025-03-17T21:09:56Z
dc.date.issued2020-04-27
dc.descriptionFunding: This research was funded by the Portuguese national budget P2020 in the scope of the project no. 023310 – ALGACO2: “Cultivo industrial de microalgas como tecnologia verde para captura de CO2 atmosférico”. This study received Portuguese national funds from FCT - Foundation for Science and Technology through project UIDB/04326/2020, and from the operational programmes CRESC Algarve 2020 and COMPETE 2020 through projects EMBRC.PT ALG-01-0145-FEDER-022121 and BIODATA.PT ALG-01-0145-FEDER-022231 and support of Foundation for Science and Technology (FCT), within the scope of the project UIDB/04292/2020 – MARE - Marine and Environmental Sciences Centre
dc.description.abstractBioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g·m-2·day-1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g·m-2·day-1). Chlorococcum amblystomatis displayed high protein content (48%-56% dry weight (DW)) and moderate levels of total lipids (18%-31% DW), carbohydrates (6%-18% DW) and ashes (9%-16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg·g-1 DW, followed by lutein with 5.37 mg·g-1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent14
dc.format.extent1228068
dc.identifier.doi10.3390/app10093040
dc.identifier.issn2076-3417
dc.identifier.otherPURE: 26688245
dc.identifier.otherPURE UUID: 3651ef8d-8835-44a0-a86e-60fc481c1bf1
dc.identifier.otherScopus: 85085499008
dc.identifier.otherWOS: 000535541900053
dc.identifier.urihttp://hdl.handle.net/10362/180750
dc.identifier.urlhttps://www.scopus.com/pages/publications/85085499008
dc.identifier.urlhttps://www.mdpi.com/2076-3417/10/9/3040
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBioprospection
dc.subjectBiotechnological applications
dc.subjectChlorococcum (Oophila) amblystomatis
dc.subjectIndustrial-scale production
dc.subjectMicroalgae
dc.subjectPhotobioreactors
dc.subjectMicrobiology
dc.subjectApplied Microbiology and Biotechnology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleIsolation, identification and biotechnological applications of a novel, robust, free-living chlorococcum (Oophila) amblystomatis strain isolated from a local ponden
dc.typejournal article
degois.publication.firstPage3040
degois.publication.issue9
degois.publication.lastPage3054
degois.publication.titleApplied Sciences
degois.publication.volume10
dspace.entity.typePublication
rcaap.rightsopenAccess

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