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Isolation, identification and biotechnological applications of a novel, robust, free-living chlorococcum (Oophila) amblystomatis strain isolated from a local pond
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Orientador(es)
Resumo(s)
Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g·m-2·day-1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g·m-2·day-1). Chlorococcum amblystomatis displayed high protein content (48%-56% dry weight (DW)) and moderate levels of total lipids (18%-31% DW), carbohydrates (6%-18% DW) and ashes (9%-16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg·g-1 DW, followed by lutein with 5.37 mg·g-1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.
Descrição
Funding: This research was funded by the Portuguese national budget P2020 in the scope of the project no. 023310 – ALGACO2: “Cultivo industrial de microalgas como tecnologia verde para captura de CO2 atmosférico”. This study received Portuguese national funds from FCT - Foundation for Science and Technology through project UIDB/04326/2020, and from the operational programmes CRESC Algarve 2020 and COMPETE 2020 through projects EMBRC.PT ALG-01-0145-FEDER-022121 and BIODATA.PT ALG-01-0145-FEDER-022231 and support of Foundation for Science and Technology (FCT), within the scope of the project UIDB/04292/2020 – MARE - Marine and Environmental Sciences Centre
Palavras-chave
Bioprospection Biotechnological applications Chlorococcum (Oophila) amblystomatis Industrial-scale production Microalgae Photobioreactors Microbiology Applied Microbiology and Biotechnology SDG 3 - Good Health and Well-being