| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.86 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
Na atualidade forense a análise de vestígios que contenham frações suficientes de conteúdo biológico, do qual seja possível extrair quantidades significativas de ADN, trata-se do primeiro obstáculo enfrentado nesta área. Para as ciências forenses a obtenção de uma amostragem rica em DNA nem sempre é a realidade observada, ainda que na maioria das vezes por onde quer que passamos possamos através do toque ou da perda de um simples cabelo, deixar para trás informação identificativa. Não obstante a existência de material biológico, distintos fatores como a degradação da amostra ou a presença de inibidores poderão colocar em causa a obtenção de resultados com valor identificativo. A quantificação do ADN é desta forma um procedimento essencial para a otimização do processo de obtenção de um perfil genético com valor identificativo. Através da técnica de quantificação de material genético qPCR, surge o novo kit Investigator Quantiplex Pro FLX (QIAGEN) com a principal vantagem de conter os seus reagentes liofilizados, quantificar ínfimas concentrações de ADN humano e especial desempenho na quantificação de ADN masculino.
Por conseguinte, o principal objetivo do presente estudo consistiu na implementação e validação deste kit no setor de biologia do Laboratório de Polícia Científica (LPC). Tendo sido utilizados dois principais grupos de amostras: amostras controlo (de modo a efetuar a validação) e amostras work case (com a finalidade de fundamentar a implementação), ambos quantificados com kit em estudo e com o kit em vigor no LPC.
Em suma, a performance dos kits em estudo não foi ao encontro dos resultados expectados. E embora o kit Investigator Quantiplex Pro FLX demonstre elevada sensibilidade na deteção de ADN masculino, não foram constatadas diferenças significativas entre este kit e o kit atualmente em uso no LPC, pelo que, a aquisição do kit Investigator Quantiplex Pro FLX não representa uma vantagem significativa.
In modern forensics, the analysis of traces containing sufficient biological content from which significant amounts of DNA can be extracted is the first obstacle faced in this area. In forensic science, obtaining a DNA-rich sample is not always a reality, even though most of the time, wherever we go, we can leave behind identifying information through touch or the loss of a single hair. Despite the existence of biological material, various factors such as sample degradation or the presence of inhibitors can jeopardize the obtaining of results with identifying value. DNA quantification is therefore an essential procedure for optimizing the process of obtaining a genetic profile with identifying value. Using the qPCR genetic material quantification technique, the new Investigator Quantiplex Pro FLX (QIAGEN) kit has emerged as the main advantage of containing its lyophilized reagents, quantifying minute concentrations of human DNA, and performing particularly well in the quantification of male DNA. Therefore, the main objective of this study was to implement and validate this kit in the biology department of the Scientific Police Laboratory (LPC). Two main groups of samples were used: control samples (for validation purposes) and work case samples (to support implementation), both quantified with the kit under study and with the kit currently in use at the LPC. In summary, the performance of the kits under study did not meet the expected results. Although the Investigator Quantiplex Pro FLX kit demonstrates high sensitivity in the detection of male DNA, no significant differences were found between this kit and the kit currently in use at the LPC, so the acquisition of the Investigator Quantiplex Pro FLX kit does not represent a significant advantage.
In modern forensics, the analysis of traces containing sufficient biological content from which significant amounts of DNA can be extracted is the first obstacle faced in this area. In forensic science, obtaining a DNA-rich sample is not always a reality, even though most of the time, wherever we go, we can leave behind identifying information through touch or the loss of a single hair. Despite the existence of biological material, various factors such as sample degradation or the presence of inhibitors can jeopardize the obtaining of results with identifying value. DNA quantification is therefore an essential procedure for optimizing the process of obtaining a genetic profile with identifying value. Using the qPCR genetic material quantification technique, the new Investigator Quantiplex Pro FLX (QIAGEN) kit has emerged as the main advantage of containing its lyophilized reagents, quantifying minute concentrations of human DNA, and performing particularly well in the quantification of male DNA. Therefore, the main objective of this study was to implement and validate this kit in the biology department of the Scientific Police Laboratory (LPC). Two main groups of samples were used: control samples (for validation purposes) and work case samples (to support implementation), both quantified with the kit under study and with the kit currently in use at the LPC. In summary, the performance of the kits under study did not meet the expected results. Although the Investigator Quantiplex Pro FLX kit demonstrates high sensitivity in the detection of male DNA, no significant differences were found between this kit and the kit currently in use at the LPC, so the acquisition of the Investigator Quantiplex Pro FLX kit does not represent a significant advantage.
Descrição
Palavras-chave
ADN qPCR Quantificação Vestígio