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Laboratory Evolution of New Bacterial Galactose Oxidases for Green Biotech Applications

datacite.subject.fosCiências Naturaispt_PT
dc.contributor.authorDias, Carolina Gomes Ferreira
dc.date.accessioned2024-02-06T23:08:20Z
dc.date.available2024-10-31T01:32:04Z
dc.date.issued2023-01-19
dc.date.submitted2022-10-01
dc.description.abstract"Galactose oxidases (GalOxs) are copper radical oxidases (CROs) that catalyze the oxidation of primary alcohols to the corresponding aldehydes, reducing molecular oxygen to hydrogen peroxide. These monomeric metalloenzymes have a copper ion as a cofactor and are secreted by filamentous fungi, with FgrGalOx being the best-studied representative. GalOxs holds potential for various biotechnological applications in small molecule synthesis, oxygen removal, and biosensors. Therefore, a better understanding of GalOx from different organisms is crucial, as it may reveal novel or improved properties to be explored in various fields. This work unravels the properties of a new bacterial galactose oxidase, AsGalOx, from Pseudoarthrobacter siccitolerans while simultaneously tailoring its properties through enzyme engineering approaches.(...)"pt_PT
dc.identifier.tid203518640pt_PT
dc.identifier.urihttp://hdl.handle.net/10362/163175
dc.language.isoengpt_PT
dc.subjectradical copper oxidasespt_PT
dc.subjectgalactose oxidasept_PT
dc.subjectdirected evolutionpt_PT
dc.subjectcatalytic efficiencypt_PT
dc.titleLaboratory Evolution of New Bacterial Galactose Oxidases for Green Biotech Applicationspt_PT
dc.typemaster thesis
dspace.entity.typePublication
rcaap.embargofctArtigos por publicarpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typemasterThesispt_PT
thesis.degree.nameDissertation presented to obtain the Master Degree in Biotechnology for Sustainabilitypt_PT

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