Publicação
Development of an Analytical Toolbox for the Assessment of Complex Biologics
| datacite.subject.fos | Ciências Naturais | pt_PT |
| dc.contributor.advisor | Alves, Patricia Gomes | |
| dc.contributor.advisor | Gomes, Ricardo | |
| dc.contributor.author | Ramos, Ana Rebeca Oliveira | |
| dc.date.accessioned | 2025-03-24T15:34:19Z | |
| dc.date.available | 2025-03-31T00:31:49Z | |
| dc.date.issued | 2022-06-02 | |
| dc.date.submitted | 2022-03-03 | |
| dc.description.abstract | "The biopharmaceutical industry is expanding rapidly and currently accounts for about 20% of the pharmaceutical market. Clinical and manufacturing processes for biologics, such as recombinant proteins or therapeutic monoclonal antibodies (mAbs), are lengthy, complex, and must meet safety and efficacy standards. As the complexity of the therapeutic products increases, the characterization becomes even more challenging, requiring the development of advanced analytical methodologies with increased sensitivity, throughput, and time/cost-effectiveness. Product heterogeneity (such as glycoforms, post-translational modifications, and sequence variants), and process-related impurities such as host cell proteins (HCP), are key product attributes that need to be monitored. ELISA-based HCP assays, commonly used for total HCP quantification, present several drawbacks, namely, not identifying individual HCPs, nor distinguishing their relative abundances.(...)" | pt_PT |
| dc.identifier.tid | 203210360 | |
| dc.identifier.uri | http://hdl.handle.net/10362/181187 | |
| dc.language.iso | eng | pt_PT |
| dc.subject | monoclonal antibodies | pt_PT |
| dc.subject | host cell protein | pt_PT |
| dc.subject | SWATH-MS | pt_PT |
| dc.subject | absolute quantification | pt_PT |
| dc.subject | average curve | pt_PT |
| dc.title | Development of an Analytical Toolbox for the Assessment of Complex Biologics | pt_PT |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.embargofct | Artigos por publicar | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | masterThesis | pt_PT |
| thesis.degree.name | Dissertation presented to obtain the Master degree in Biochemistry for Health | pt_PT |