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SARS-CoV-2 Spike and Receptor Binding Domain Glycoproteins Produced in Tobacco BY-2 and Medicago Cell Suspension Cultures

2022-12-02Dissertação de mestrado Acesso aberto
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datacite.subject.fosCiências Naturaispt_PT
dc.contributor.advisorAbranches, Rita Sobral
dc.contributor.authorFerreira, Ana Clara
dc.date.accessioned2025-03-24T18:09:40Z
dc.date.available2025-10-31T01:31:01Z
dc.date.issued2022-12-02
dc.date.submitted2022-11-04
dc.description.abstract"The rapid worldwide spread of SARS-CoV-2 has motivated the scientific community to develop efficient and specific diagnostics and therapies in response to the COVID-19 pandemic. A variety of approaches have been considered, including the production of COVID-related proteins, with the Spike and its RBD as the major targets for expression in various systems. RBD and Spike protein production have previously been achieved in eukaryotes, namely animal cell cultures and via transient expression in plants. Spike protein production in microbial systems has been unsuccessful until now. In this dissertation, an alternative eukaryotic system was used for the production of SARS-CoV-2 Spike and RBD. The effective production of these viral proteins was successfully achieved in two plant cell suspension cultures: Tobacco BY-2 and Medicago truncatula A17 cell lines.(...)"pt_PT
dc.identifier.tid203208641pt_PT
dc.identifier.urihttp://hdl.handle.net/10362/181201
dc.language.isoengpt_PT
dc.subjectstable transformationpt_PT
dc.subjectrecombinant viral proteinpt_PT
dc.subjectMedicago truncatulapt_PT
dc.subjectplant cell cultures, COVID-19,pt_PT
dc.subjecttobacco BY-2 cellspt_PT
dc.titleSARS-CoV-2 Spike and Receptor Binding Domain Glycoproteins Produced in Tobacco BY-2 and Medicago Cell Suspension Culturespt_PT
dc.typemaster thesis
dspace.entity.typePublication
person.familyNameBrito Ferreira
person.givenNameAna Clara
person.identifier.ciencia-id1F15-4B09-C1F3
person.identifier.orcid0000-0002-5011-8993
rcaap.embargofctArtigos por publicarpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typemasterThesispt_PT
relation.isAuthorOfPublication5871a224-88cc-43e1-81ca-cffd9cb3ea2b
relation.isAuthorOfPublication.latestForDiscovery5871a224-88cc-43e1-81ca-cffd9cb3ea2b
thesis.degree.nameDissertation presented to obtain the Master degree in Biochemistry for Healthpt_PT

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ITQB: PCB - MA Dissertations