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Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates

2026-05Artigo científico Acesso aberto
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dc.contributor.authorBogojevic, Sanja Skaro
dc.contributor.authorPantelic, Brana
dc.contributor.authorPonjavic, Marijana
dc.contributor.authorIlic-Tomic, Tatjana
dc.contributor.authorMilivojevic, Dusan
dc.contributor.authorGuzik, Maciej
dc.contributor.authorRychwalski, Marcin
dc.contributor.authorSiaperas, Romanos
dc.contributor.authorTopakas, Evangelos
dc.contributor.authorCarvalheira, Mónica
dc.contributor.authorFreitas, Filomena
dc.contributor.authorConcórdio-Reis, Patrícia
dc.contributor.authorNikodinovic-Runic, Jasmina
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionFaculdade de Ciências e Tecnologia (FCT)
dc.contributor.pblBlackwell
dc.date.accessioned2026-06-22T12:25:05Z
dc.date.available2026-06-22T12:25:05Z
dc.date.issued2026-05
dc.descriptionPublisher Copyright: © 2026 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.
dc.description.abstractPolyhydroxyalkanoates (PHAs) are intracellular microbial polyesters whose commercial deployment is strongly influenced by downstream processing costs and environmental burden. Here, we demonstrate a nearly solvent-free, mild aqueous strategy for the recovery of PHAs directly from wet bacterial biomass using crude enzyme systems derived from Tenebrio molitor. Under optimized conditions (0.6 wt% crude protein, pH 7.6, 40°C), near-quantitative (≥ 95%) recovery of PHB and PHB/HV and up to 60% recovery of mcl-PHA were achieved without prior biomass drying. Proteomic analysis identified abundant digestive hydrolases, including α-amylase and cathepsin and recombinant validation confirmed their contribution to polymer release, demonstrating that targeted enzyme combinations can substantially enhance mcl-PHA recovery (up to 94%–98%). Gel permeation chromatography, NMR and thermal analysis demonstrated preservation of polymer molecular integrity and crystallinity comparable to chloroform extraction. Life cycle assessment revealed a three- to seven-fold reduction in carbon footprint relative to conventional solvent and classical enzymatic methods, primarily due to elimination of drying and solvent use. These findings establish biologically driven biomass hydrolysis as a scalable downstream strategy and highlight insect-derived enzyme systems as promising tools for integrated microbial biopolymer processing.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent895137
dc.identifier.doi10.1111/1751-7915.70360
dc.identifier.issn1751-7907
dc.identifier.otherPURE: 165272855
dc.identifier.otherPURE UUID: f8519d37-094f-42d8-8fe4-20d698aa4ad8
dc.identifier.otherScopus: 105037562571
dc.identifier.otherPubMed: 42059372
dc.identifier.otherWOS: 001753595000001
dc.identifier.otherORCID: /0000-0002-9430-4640/work/218473629
dc.identifier.otherORCID: /0000-0002-3862-2636/work/218473655
dc.identifier.urihttp://hdl.handle.net/10362/203979
dc.identifier.urlhttps://www.scopus.com/pages/publications/105037562571
dc.language.isoeng
dc.peerreviewedyes
dc.subjectbacterial biomass
dc.subjectbioplastic
dc.subjectcarbon footprint
dc.subjectdownstream processing
dc.subjectenzyme
dc.subjectpolyhydroxyalkanoates
dc.subjectTenebrio molitor
dc.subjectBiotechnology
dc.subjectBioengineering
dc.subjectBiochemistry
dc.subjectApplied Microbiology and Biotechnology
dc.subjectSDG 7 - Affordable and Clean Energy
dc.subjectSDG 12 - Responsible Consumption and Production
dc.titleTenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoatesen
dc.typejournal article
degois.publication.issue5
degois.publication.titleMicrobial Biotechnology
degois.publication.volume19
dspace.entity.typePublication
rcaap.rightsopenAccess

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