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Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates
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Orientador(es)
Resumo(s)
Polyhydroxyalkanoates (PHAs) are intracellular microbial polyesters whose commercial deployment is strongly influenced by downstream processing costs and environmental burden. Here, we demonstrate a nearly solvent-free, mild aqueous strategy for the recovery of PHAs directly from wet bacterial biomass using crude enzyme systems derived from Tenebrio molitor. Under optimized conditions (0.6 wt% crude protein, pH 7.6, 40°C), near-quantitative (≥ 95%) recovery of PHB and PHB/HV and up to 60% recovery of mcl-PHA were achieved without prior biomass drying. Proteomic analysis identified abundant digestive hydrolases, including α-amylase and cathepsin and recombinant validation confirmed their contribution to polymer release, demonstrating that targeted enzyme combinations can substantially enhance mcl-PHA recovery (up to 94%–98%). Gel permeation chromatography, NMR and thermal analysis demonstrated preservation of polymer molecular integrity and crystallinity comparable to chloroform extraction. Life cycle assessment revealed a three- to seven-fold reduction in carbon footprint relative to conventional solvent and classical enzymatic methods, primarily due to elimination of drying and solvent use. These findings establish biologically driven biomass hydrolysis as a scalable downstream strategy and highlight insect-derived enzyme systems as promising tools for integrated microbial biopolymer processing.
Descrição
Publisher Copyright: © 2026 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.
Palavras-chave
bacterial biomass bioplastic carbon footprint downstream processing enzyme polyhydroxyalkanoates Tenebrio molitor Biotechnology Bioengineering Biochemistry Applied Microbiology and Biotechnology SDG 7 - Affordable and Clean Energy SDG 12 - Responsible Consumption and Production