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Projeto de investigação
Soluções eco-inovadoras para adaptação da aquacultura aos desafios das alterações climáticas: uma abordagem multi-ómica integrada para avaliar as respostas imuno-metabólicas dos peixes marinhos
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Eco-innovative aquafeeds biofortified with Asparagopsis taxiformis to improve the resilience of farmed white seabream (Diplodus sargus) to marine heatwave events
Publication . Marmelo, Isa; Lourenço-Marques, Cátia; Silva, Iris A.L.; Soares, Florbela; Pousão-Ferreira, Pedro; Mata, Leonardo; Marques, António; Diniz, Mário Sousa; Maulvault, Ana Luísa; UCIBIO - Applied Molecular Biosciences Unit; Faculdade de Ciências e Tecnologia (FCT); Elsevier
Extreme weather events, like marine heatwaves (MHWs), are becoming more frequent and severe due to climate change, posing several challenges to marine ecosystems and their services. As disease outbreaks are often prompted by these acute phenomena, it is essential to develop eco-innovative strategies that can efficiently improve farmed fish resilience, especially under sub-optimal rearing conditions, thereby ensuring a sustainable aquaculture production. This study aimed to unveil farmed juvenile white seabream (Diplodus sargus, 28.50 ± 1.10 g weight, n = 150) immune and antioxidant responses under a category II MHW in the Mediterranean Sea (+4 °C, 8 days of temperature increase plus 15 days of plateau at the peak temperature) and to investigate whether a 30 days period of prophylactic biofortification with Asparagopsis taxiformis (1.5 %, 3 % and 6 %) enhanced fish resilience to these extreme events. Several biomarkers from different organization levels (individual, cellular, biochemical and molecular) were assessed upon 30 days of biofortification (T30), exposure (after 8 days of temperature increase + 15 days at peak temperature, T53) and recovery (8 days of temperature decrease, T61) from the MHW. Results showed that MHW negatively affected the fish physiological status and overall well-being, decreasing specific growth rate (SGR) and haematocrit (Ht) and increasing erythrocyte nuclear abnormalities (ENAs) and lipid peroxidation (LPO). These adverse effects were alleviated through biofortification with A. taxiformis. Seaweed inclusion at 1.5 % was the most effective dose to minimize the severity of MHW effects, significantly improving immune responses of D. sargus (i.e. increased levels of immunoglobulin M, peroxidase activity and lysozyme expression) and modulating antioxidant responses (i.e. decreased LPO, catalase and glutathione S-transferase activity). These findings confirm that A. taxiformis is a functional ingredient of added value to the aquaculture industry, as its inclusion in marine fish diets can beneficially modulate fish immunity and resilience under optimal and adverse rearing conditions.
Asparagopsis taxiformis Feed Supplementation as a Tool to Improve the Resilience of Farmed Diplodus sargus to Marine Heatwave Events—A Metabolomics Approach
Publication . Dias, Marta; Marmelo, Isa; António, Carla; Rodrigues, Ana M.; Marques, António; Diniz, Mário S.; Maulvault, Ana Luísa; UCIBIO - Applied Molecular Biosciences Unit; Faculdade de Ciências e Tecnologia (FCT); DQ - Departamento de Química; MDPI - Multidisciplinary Digital Publishing Institute
The need to maximize aquaculture production while addressing environmental and food security challenges posed by climate change has driven research towards the development of functional aquafeeds that enhance performance and immunity in farmed species. However, exposure to dietary and environmental stressors affects marine organisms, altering key metabolic pathways best understood through high-throughput “omics” tools. This study assessed the effects of Asparagopsis taxiformis supplementation on central metabolic pathways by analyzing changes in primary metabolite levels in the liver of farmed Diplodus sargus under optimal and suboptimal temperature conditions. Results showed that seaweed supplementation had a beneficial effect on the fish’s primary metabolome; however, inclusion levels and rearing conditions played a crucial role in determining outcomes. While 1.5% supplementation maintained a balanced primary metabolome under optimal temperature conditions, 3.0% supplementation most effectively mitigated the adverse effects of acute thermal stress during a marine heatwave. These findings highlight the nutritive and functional potential of A. taxiformis supplementation in aquafeeds for marine omnivorous fish species and emphasize the importance of evaluating functional aquafeeds under suboptimal rearing conditions. Overall, our results demonstrate the value of metabolomics in elucidating the molecular basis underlying biological pathways in farmed marine fish and optimizing production through climate-smart dietary strategies.
Hot and toxic
Publication . Dias, Marta; Özkan, Busenur; Ramos, João; Marques, António; Rosa, Rui; Costa, Pedro Reis; Maulvault, Ana Luísa; UCIBIO - Applied Molecular Biosciences Unit; MARE - Centro de Ciências do Mar e do Ambiente; Elsevier
Climate change is increasing marine heatwaves (MHWs) frequency and severity worldwide. These extreme events often cause bivalves' mass mortality and facilitate the growth, proliferation and dispersion of toxin-producing microalgae blooms associated with threats to seafood safety. Yet, the interactive effects between MHW and uptake of marine biotoxins by biota are a novel topic still lacking thorough research, from both the ecotoxicological and seafood safety standpoints. This study assessed the effects of a MHW event on the accumulation/elimination dynamics of diarrhetic shellfish toxins in Mytilus galloprovincialis exposed to Prorocentrum lima and the ecotoxicological responses of mussels co-exposed to these two stressors. Results showed that acute exposure to +4 °C reduced toxins accumulation (−49 %) and elimination (−77 %) compared to control temperature. Moreover, exposure to MHW and toxins affected mussels' antioxidant activity, lipid and protein damage, and metabolism in a tissue-specific manner. These findings highlight that M. galloprovincialis can face higher vulnerability to toxins when MHW events strike.
Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure
Publication . Gomes, Rita V. C.; Borrull, Sílvia; Pereira, Alícia; Dias, Marta; Cereja, Rui; Barata, Marisa; Pousão-Ferreira, Pedro; Faria, Ana M.; Pocurull, Eva; Marcé, Rosa Maria; Marques, António; Maulvault, Ana Luísa; UCIBIO - Applied Molecular Biosciences Unit; Elsevier
High Production Volume Chemicals (HPVCs) are contaminants that pose serious threats to aquatic environments and species that inhabit them, given their massive production and ubiquitous distribution across biological compartments. Among them, organophosphate esters (OPEs) are of particular concern, as they are widely used as plasticizers and flame-retardants, and linked to various forms of toxicity in marine organisms. In this study, we investigated the ecotoxicological response of juvenile gilthead seabream Sparus aurata to the OPE tris (2-chloroisopropyl) phosphate (TCPP) following chronic dietary exposure to three different concentrations (low, D1: 0.2 mg kg−1; ecologically relevant, D2: 2 mg kg−1; and high, D3: 10 mg kg−1). Different biomarkers indicative of antioxidant defence mechanisms (catalase, CAT, glutathione S-transferase, GST, activities), metabolism (citrate synthase, CS, lactate dehydrogenase, LDH, activities) and endocrine disruption (vitellogenin content, VTG), as well as cell (lipid peroxidation levels, LPO) and protein damage (ubiquitin content, UBI) were analyzed in liver and muscle to assess TCPP toxicity. High concentrations of TCPP affected S. aurata growth, but not overall fitness condition. Furthermore, metabolic disruption and severe oxidative damages were observed, regardless of exposure dose. VTG content significantly decreased after exposure to all TCPP dosages, indicating a possible masculinization effect. These findings provide new insights to the scientific knowledge on TCPP ecotoxicological attributes and impacts on marine ichthyofauna. In addition, our results confirm the relevance of conducting integrated multi-biomarker approaches to disclose the ecotoxicological effects of poorly studied chemical contaminants and, ultimately, implement wastewater treatment strategies and legislation to protect marine ecosystems from pollution.
Asparagopsis taxiformis as a Novel Antioxidant Ingredient for Climate-Smart Aquaculture
Publication . Pereira, Alícia; Marmelo, Isa; Dias, Marta; Silva, Ana Catarina; Grade, Ana Catarina; Barata, Marisa; Pousão-Ferreira, Pedro; Dias, Jorge; Anacleto, Patrícia; Marques, António; Diniz, Mário S.; Maulvault, Ana Luísa; UCIBIO - Applied Molecular Biosciences Unit; Faculdade de Ciências e Tecnologia (FCT); MDPI - Multidisciplinary Digital Publishing Institute
The increasing frequency and duration of marine heatwaves (MHWs) due to climate change pose severe threats to aquaculture, causing drastic physiological and growth impairments in farmed fish, undermining their resilience against additional environmental pressures. To ensure sustainable production that meets the global seafood demand and animal welfare standards, cost-effective and eco-friendly strategies are urgently needed. This study explored the efficacy of the red macroalga Asparagopsis taxiformis on juvenile white seabream Diplodus sargus reared under optimal conditions and upon exposure to a MHW. Fish were fed with four experimental diets (0%, 1.5%, 3% or 6% of dried powdered A. taxiformis) for a prophylactic period of 30 days (T30) and subsequently exposed to a Mediterranean category II MHW for 15 days (T53). Biometric data and samples were collected at T30, T53 and T61 (8 days post-MHW recovery), to assess performance indicators, biomarker responses and histopathological alterations. Results showed that A. taxiformis supplementation improved catalase and glutathione S-transferase activities and reduced lipid peroxidation promoted by the MHW, particularly in fish biofortified with 1.5% inclusion level. No histopathological alterations were observed after 30 days. Additionally, fish biofortified with 1.5% A. taxiformis exhibited increased citrate synthase activity and fish supplemented with 1.5% and 3% showed improved digestive enzyme activities (e.g., pepsin and trypsin activities). Overall, the present findings pointed to 1.5% inclusion as the optimal dosage for aquafeeds biofortification with A. taxiformis, and confirmed that this seaweed species is a promising cost-effective ingredient with functional properties and great potential for usage in a climate-smart context.
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Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
3599-PPCDT
Número da atribuição
PTDC/CTA-AMB/0592/2021