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Projeto de investigação
Bioresources 4 Sustainability
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Primary metabolite profile changes in coffea spp. Promoted by single and combined exposure to drought and elevated co2 concentration
Publication . Rodrigues, Ana M.; Jorge, Tiago; Osório, Sónia; Pott, Delphine M.; Lidon, Fernando C.; Damatta, Fábio M.; Marques, Isabel; Ribeiro-Barros, Ana I.; Ramalho, José C.; António, Carla; Instituto de Tecnologia Química e Biológica António Xavier (ITQB); GeoBioTec - Geobiociências, Geoengenharias e Geotecnologias; DCT - Departamento de Ciências da Terra; MDPI - Multidisciplinary Digital Publishing Institute
Climate change scenarios pose major threats to many crops worldwide, including coffee. We explored the primary metabolite responses in two Coffea genotypes, C. canephora cv. Conilon Clone 153 and C. arabica cv. Icatu, grown at normal (aCO2) or elevated (eCO2) CO2 concentrations of 380 or 700 ppm, respectively, under well-watered (WW), moderate (MWD), or severe (SWD) water deficit conditions, in order to assess coffee responses to drought and how eCO2 can influence such responses. Primary metabolites were analyzed with a gas chromatography time-of-flight mass spectrometry metabolomics platform (GC-TOF-MS). A total of 48 primary metabolites were identified in both genotypes (23 amino acids and derivatives, 10 organic acids, 11 sugars, and 4 other metabolites), with differences recorded in both genotypes. Increased metabolite levels were observed in CL153 plants under single and combined conditions of aCO2 and drought (MWD and SWD), as opposed to the observed decreased levels under eCO2 in both drought conditions. In contrast, Icatu showed minor differences under MWD, and increased levels (especially amino acids) only under SWD at both CO2 concentration conditions, although with a tendency towards greater increases under eCO2. Altogether, CL153 demonstrated large impact under MWD, and seemed not to benefit from eCO2 in either MWD and SWD, in contrast with Icatu.
Unravelling how the biosynthesis of sphingolipids impacts stress responses in Aspergillus nidulans
Publication . Piontkivska, Daryna; Pereira, Cristina Silva
"For the past years filamentous fungi have been emerging as critical human-pathogens and have been increasingly provoking deadly invasive infections. The currently available antifungal drugs are inadequate to fight this global threat and the discovery of new antifungal targets are urgently needed. In this context, fungal sphingolipids have come up as potential targets for the development of new antifungal agents; however, knowledge on sphingolipid biosynthetic pathways in filamentous fungi, such as Aspergillus nidulans, is still rather limited. Previous observations demonstrated that some ionic liquids can trigger the accumulation of sphingolipid intermediates of the glucosylceramide biosynthetic pathway, that in response to the induced stress are probably acting as second messengers. In the present thesis we wanted to assess how sphingolipid biosynthesis can impact stress responses in A. nidulans.(...)"
Elucidate the biosy nthesis and the functional role of a new class of antimicrobial peptaibiotics in Neurospora crassa
Publication . Sequeira, Patrícia Alexandra Soares
"Fungi are generally abundant producers of secondary metabolites. In particular, these organisms are involved in the production of a subclass of nonribosomal peptides - peptaibiotics - which display interesting antibiotic activities. Previous studies demonstrated that some fungal strains are able to grow in media supplemented with ionic liquid and this supplementation leads to alterations in their metabolic footprint. Moreover, in Neurospora crassa, growth media supplementation with either 1-ethyl-3-methylimidazolium chloride or cholinium chloride, led to increased levels of 1-aminocyclopropane-1-carboxylate deaminase, an enzyme involved in the production of the rare amino acid 1-aminocyclopropane-1-carboxylic acid (ACC).(...)"
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Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
6817 - DCRRNI ID
Número da atribuição
UID/Multi/04551/2013