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Production of marine microbial collagenases in cultured plant cells
Publication . Santos, Sofia Rebelo Azevedo dos; Abranches, Rita; Moe, Elin
The fishing and seafood industries play a key role in the Portuguese economy. However, the by products of these industries are currently discarded, despite their high potential for the production of valuable compounds due to their high collagen content. Collagen can be degraded by specific enzymes, known as collagenases, into small bioactive compounds called collagen peptides, which have gained considerable attention for their apparent skin rejuvenating and antioxidant effects. These collagenases, particularly marine bacterial collagenases, are highly complex enzymes with the potential to exhibit novel properties. Recombinant production of collagenases in bacterial systems has been previously demonstrated; however, they often accumulate in insoluble forms, posing a significant challenge for their purification. This thesis proposes an alternative approach, namely the development of a sustainable platform for the production of collagenases using plant cell suspension cultures, a simple and inexpensive eukaryotic system. Following codon optimization and successful cloning of a collagenase sequence into a suitable vector, plant cell cultures of Medicago truncatula A17 and Tobacco BY-2 were established by Agrobacterium tumefaciens-mediated transformation. Successful production of the recombinant collagenase was demonstrated in the cell extract of the tobacco cell lines, indicating that plant cells may serve as a viable alternative for the production of soluble collagenases, providing a sustainable and scalable platform with significant economic and environmental implications. By valorising collagen rich fishery by-products, this approach could contribute to a circular economy.
2024-12-03Dissertação de mestrado Acesso embargado Ver mais

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Fundação para a Ciência e a Tecnologia

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Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Base

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UIDB/04551/2020

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