ITQB: BL-PhD Thesis
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- Structurally Primed Phage display LibrariesPublication . Moura-Sampaio, Jorge"Therapeutic monoclonal antibodies (mAbs) are one of the main drivers of revenue of the pharmaceutical market. Regardless of the origin and platform used, monoclonal antibodies generated against a given target may have room for improvement. Using in vitro affinity maturation libraries aims to surpass the throughput limitations of classical X-ray crystallography affinity maturation approaches, by providing a generalizable approach (or blind) that can be applied to many candidates. The current blind methods do not always assure that synergistic mutations are found and may not respect the structural constraints of the IgG molecule in question. Ideally, innovative affinity maturation methods should be generalizable to provide high-throughput results while maintaining a certain degree of specificity towards the antibody structure being considered. As such they require attention to be paid to specific regions, such as the ones likely to be in contact with the antigen, or regions that influence the antibodies’ structural integrity and overall developability.(...)"