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Quantum dots: synthesis, functionalization and bioconjugation for biological applications
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The main goal of this Doctoral work was to develop a fluorescent biomarker to
identify antigenic proteins associated with specific parasites. Although it is
known that fluorescent techniques making use of standard organic dyes are
widely used for this purpose, the development of a new method was proposed
using nanotechnology; in particular the use of nano optical reporters also
called quantum dots (QDs). In order to achieve this goal, the research work
was divided into four main tasks: (1) synthesis and characterization of
CdSe/ZnS core-shell QDs; (2) design of dihydrolipoic acid (DHLA) ligands
appended to oligo and poly (ethylene) glycols (PEG) with different functional
groups to generate biocompatible QDs; (3) bioconjugation of QDs to
monoclonal antibodies based on sophisticated protocols and (4) some
chemical and biological applications of the synthesized non-conjugated and
conjugated nanoparticles.(...)
Descrição
Dissertation presented to obtain the Ph.D degree in Engineering Sciences
and Technology
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Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica