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Dynamics of cork mycobiota throughout stopper manufacturing process: from diversity to metabolite
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Cork, the continuous layer of outer bark of the Quercus suber L.
tree, has physical and chemical properties that are unique.
Portugal possesses 33 % of the world’s cork oak forests and
accounts for approximately half of total global cork production.
The manufacture of cork discs (or stoppers) comprises several
stages, including two boiling stages, during which slabs of cork
are steeped in boiling water. In days following the boiling the
humidity of the slabs decreases and they become completely
covered in a white mycelium of Chrysonilia sitophila until the cork
achieves a certain water activity level (ca 0.9 aw). Below this
level other fungal species (e.g. Penicillium, Aspergillus or
Trichoderma) can germinate and shift the fungal colonization of
the cork slabs.
The two main objectives of the research described in the
presented PhD thesis are (1) a taxonomic identification of the
mycobiota present in cork slabs throughout the manufacture of
cork discs, and (2) an investigation into the chemical compounds,
which can give unfavourable properties to the cork, produced by these fungi.(...)
Descrição
Dissertation presented to obtain the Ph.D degree in Biology
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Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica