Digital microfluidics for nucleic acid amplification

Coelho, Beatriz; Veigas, Bruno; Fortunato, Elvira; Martins, Rodrigo; Águas, Hugo; Igreja, Rui; Baptista, Pedro V.

http://hdl.handle.net/10362/63112

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Resumo(s)

Digital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical reactions at a much smaller scale. DMF devices open entirely new and promising pathways for multiplex analysis and reaction occurring in a miniaturized format, thus allowing for healthcare decentralization from major laboratories to point-of-care with accurate, robust and inexpensive molecular diagnostics. Here, we shall focus on DMF platforms specifically designed for nucleic acid amplification, which is key for molecular diagnostics of several diseases and conditions, from pathogen identification to cancer mutations detection. Particular attention will be given to the device architecture, materials and nucleic acid amplification applications in validated settings.

Descrição

This work was funded by FEDER funds through the COMPETE 2020 Program (POCI-01-0145-FEDER-007728). BV thanks SFRH/BPD/124311/2016. PVB thanksWater JPI Proj TRACE (Ref 281715).

Palavras-chave

Digital microfluidics Nucleic acid amplification Point-of-care diagnostics Analytical Chemistry Atomic and Molecular Physics, and Optics Biochemistry Instrumentation Electrical and Electronic Engineering SDG 3 - Good Health and Well-being