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Publicação
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis
Orientador(es)
Resumo(s)
Background: The methicillin-resistant Staphylococcus aureus clone USA300
contains a novel mobile genetic element, arginine catabolic mobile element
( ACME), that contributes to its enhanced capacity to grow and survive within
the host. Although ACME appears to have been transferred into USA300 from S.
epidermidis, the genetic diversity of ACME in the latter species remains poorly
characterized. Methodology/Principal Findings: To assess the prevalence and
genetic diversity of ACME, 127 geographically diverse S. epidermidis isolates
representing 86 different multilocus sequence types (STs) were characterized.
ACME was found in 51% (65/127) of S. epidermidis isolates. The vast majority
(57/65) of ACME-containing isolates belonged to the predominant S. epidermidis
clonal complex CC2. ACME was often found in association with different allotypes
of staphylococcal chromosome cassette mec (SCCmec) which also encodes the
recombinase function that facilities mobilization ACME from the S. epidermidis
chromosome. Restriction fragment length polymorphism, PCR scanning and DNA
sequencing allowed for identification of 39 distinct ACME genetic variants that
differ from one another in gene content, thereby revealing a hitherto
uncharacterized genetic diversity within ACME. All but one ACME variants were
represented by a single S. epidermidis isolate; the singular variant, termed
ACME-I.02, was found in 27 isolates, all of which belonged to the CC2 lineage.
An evolutionary model constructed based on the eBURST algorithm revealed that
ACME-I.02 was acquired at least on 15 different occasions by strains belonging
to the CC2 lineage. Conclusions/Significance: ACME-I.02 in diverse S.
epidermidis isolates were nearly identical in sequence to the prototypical ACME
found in USA300 MRSA clone, providing further evidence for the interspecies
transfer of ACME from S. epidermidis into USA300.
Descrição
PLos One, 4(11): ARTe7722
Palavras-chave
ACME-ARCA GENE RESISTANCE TYPING METHODS AUREUS SEQUENCE CLONE IDENTIFICATION COMBINATION VIRULENCE USA300
Contexto Educativo
Citação
Editora
Public Library of Science