Light activation of transcription: photocaging of nucleotides for control over RNA polymerization

Pinheiro, André Vidal; Baptistap, Pedro; Lima, João Carlos

http://hdl.handle.net/10362/58207

Utilize este identificador para referenciar este registo.

Nome:	Descrição:	Tamanho:	Formato:
Light_activation_of_transcription_photocaging_of_nucleotides_for_control_over_RNA_polymerization.pdf		2.87 MB	Adobe PDF	Ver/Abrir

Contacte-nos

Autores

Pinheiro, André Vidal

Baptistap, Pedro

Lima, João Carlos

Resumo(s)

We describe the use of ATP caged with [7-(diethylamino)coumarin-4-yl]methyl (DEACM) for light-controlled in vitro transcription reactions. Polymerization is blocked when DEACM is bonded to the gamma phosphate group of the ATP molecule. Controlled light irradiation releases ATP and transcription is initiated. In order to provide full control over the process, conditions involved in substrate release, nucleotide availability after release and the effect of the released coumarin in RNA polymerization were assessed in further detail. Together, our data provide the first direct evidence of control over enzymatic polymerization of nucleic acids through light. This approach may provide researchers with a unique tool for the study of biological processes at a molecular level.

Descrição

Fundação Calouste Gulbenkian (Ref. 76436). Funding to pay the Open Access publication charges for this article was provided by Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa.