ITQB: CLDMB - PhD Theses
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- Cell-based and cell-free strategies for cardiac repairPublication . Louro, Ana; Serra, Margarida"Extensive work has been developed in the field of regenerative medicine towards addressing the fundamental mechanisms of loss of cardiac and vascular function in cardiovascular disease (CVD), particularly in heart failure. Therapies may tackle different phases and molecular processes over the course of post-insult cardiac tissue healing, either by modulating pathological responses and boosting endogenous repair pathways or by directly replacing injured areas with contractile macro tissues. (...)"
- Improving γ-retrovirus and lentivirus gene therapy vectorsPublication . Oliveira, Ana; Coroadinha, Ana Sofia de Sousa Valente"In the last decade gene therapy became an effective form of treatment for several disease indications. Successful clinical trials saved the lives of many patients otherwise with untreatable diseases and numerous products were granted market approval. Gammaretroviral and lentiviral vectors (γ- RV and LV) are the gene delivery vehicles with higher representation in clinical trials and commercialized virus-based products. (...)"
- Cell-based switch-on sensors for detection and quantification of label-free virusesPublication . Guerreiro, M.R.; Coroadinha, Ana SofiaViruses are major pathogenic agents, claiming millions of human lives every year. While immunization and antiviral drugs were able to reduce global mortality rates, effective prevention and treatment are still to be developed for several infectious diseases. In parallel, advancements on cell culture and molecular biology techniques created a new paradigm. Virus-based biopharmaceuticals are now commonly used in multiple applications, from vaccination to gene and oncolytic therapies. One major bottleneck in research in basic and applied virology is the lack of fast, reliable, and biologically relevant methodologies to detect and quantify viruses. Most of the current methods provide indirect measurements of infectious particles, over-estimating virus infectivity.(...)
- Bioprocess design of canine adenovirus vectors for gene therapy applicationsPublication . Fernandes, Paulo David; Alves, Paula; Coroadinha, Ana SofiaThe potential of human adenovirus vectors as vehicles for gene transfer with clinical applications in vaccination, cancer treatment and in many monogenic and acquired diseases has been demonstrated in several studies and clinical trials. However, the clinical use of these vectors can be limited by pre-existing humoral and cellular anti-capsid immunity. One way to circumvent this bottleneck while keeping the advantages of using adenovirus vectors is using non-human viruses such as Canine Adenovirus type 2 (CAV-2). Moreover, CAV-2 vectors present attractive features to develop potential treatment of neurodegenerative and ocular disorders. While the interest in CAV-2 vectors increases, scalable and robust production processes are required to meet the need for preclinical and possibly clinical uses.(...)
- Integrated functional genomics for improved manufacture of recombinant enveloped virusPublication . Rodrigues, Ana Filipa; Coroadinha, Ana SofiaBiopharmaceuticals derived from recombinant viruses comprise a plethora of prophylactic and therapeutic products from vaccines to gene therapy vectors. Retroviruses occupy an important segment of this market. Replication-defective vectors have extensively been used as delivery vehicles in gene therapy protocols. Retrovirus-like-particles have shown promising results in vaccinology as immunogen display platforms. Replication-competent particles have efficiently been used as oncolytic agents. (...)
- Valorization of traditional portuguese apples and cherries: biochemical characterization and development of functional ingredientsPublication . Serra, Ana Teresa de Carvalho Negrão; Duarte, Catarina M.; Alves, Paula M.The consumption of fruits and vegetables is widely recognized to be beneficial to human health and this is mainly attributed to their high content in functional constituents, such as vitamins, carotenoids and polyphenols. In Portugal, the annual production of apples and cherries is about 300,000 and 17,000 ton, respectively. Between all varieties produced, there are some traditional fruits, which are only cultivated in specific regions of the country. In general, these traditional varieties are very appreciated by consumers due to their unique sensorial properties. However, they are less produced and thus more expensive than exotic cultivars. Within this context, this thesis focused on the valorization of these traditional Portuguese fruits in order to increase their productivity and competition in the market.(...)